Toyomoto Daiki, Shibata Yukika, Uemura Masato, Taura Satoru, Sato Tadashi, Henry Robert, Ishikawa Ryuji, Ichitani Katsuyuki
United Graduate School of Agricultural Sciences, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan.
Graduate school of Agriculture, Forestry and Fisheries, Kagoshima University, 1-21-24 Korimoto, Kagoshima, Kagoshima 890-0065, Japan.
Breed Sci. 2024 Apr;74(2):146-158. doi: 10.1270/jsbbs.23084. Epub 2024 Apr 2.
Seed development is an essential phenomenon for all sexual propagative plant species. The functional allele at () or () loci is essential for seed development for and . In the present study, we performed fine mapping of , narrowing down the area of interest to 333kb on chromosome 6. Haplotype analysis around the locus of accessions indicated that they shared the DNA polymorphism, suggesting that they have a common abortive allele at the locus. Linkage analysis of the candidate gene showed that it was located on chromosome 4. The candidate was confirmed using a population in which both the and genes were segregating. The chromosomal region covering the gene was predicted to contain 30 protein-coding genes in . Five of these genes have conserved DNA sequences in the chromosomal region of the gene on chromosome 4, and not on chromosome 6, of . These results suggest that these five genes could be candidates for , and that their orthologous genes located on chromosome 4 of could be candidates for .
种子发育是所有有性繁殖植物物种的一个重要现象。（）或（）位点的功能等位基因对和的种子发育至关重要。在本研究中，我们对进行了精细定位，将感兴趣的区域缩小到6号染色体上的333kb。对种质资源位点周围的单倍型分析表明，它们共享DNA多态性，这表明它们在该位点有一个共同的败育等位基因。候选基因的连锁分析表明它位于4号染色体上。使用一个和基因均分离的群体对候选进行了验证。预测覆盖基因的染色体区域在中包含30个蛋白质编码基因。其中五个基因在的4号染色体上的基因染色体区域具有保守的DNA序列，而在6号染色体上则没有。这些结果表明，这五个基因可能是的候选基因，并且它们位于的4号染色体上的直系同源基因可能是的候选基因。
