Ziari Katayoun, Sanjari Mojgan, Safavi Moeinadin
Dept. of Pathology, AJA University of Medical Sciences, Tehran, Iran.
Endocrinology and metabolism Research Center, institute of Basic and clinical physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran.
Iran J Pathol. 2018 Spring;13(2):151-156. Epub 2018 Jul 17.
BACKGROUND & OBJECTIVE: papillary thyroid cancer is the most common cancer of thyroid accounting for 75%-85% of all thyroid malignancies. Recently, β-catenin has been determined to play a role in clinical course of human epithelial cancers. This study was designed to reveal the association of β-catenin marker and papillary thyroid carcinoma behavior.
63 paraffin blocks of papillary thyroid carcinoma were stained with ready to use monoclonal β-catenin antibody according to manufacturer's instructions. Memberanous, cytoplasmic and nuclear staining was scored according to intensityof immunoreactivity. β-catenin immunostaining association with clinical parameters like number of recurrences and cumulative dose of radioiodine therapy were analyzed using SPSS version 15. Histopathologic parameters like tumor stage, grade, capsular invasion, lymphovascular invasion, lymph node involvement, distant metastasis andother variables were also evaluated for association with β-catenin immunoreactivity.
77.8% of papillay thyroid carcinoma were well differentiated and the remaining were poorly differentiated. Loss of β-catenin membrane immunostaining depicted correlation with number of recurrences (=0.023% , Pearson correlation=-0.285). Its loss of memberanous staining correlated similarly with cumulative dose of radioiodine (= 0.046, Pearson correlation = -0.253). Loss of membranous β-catenin was significantly associated with some histopathologic findings like nodal involvement (<0.001), distant metastasis (=0.003) and tumor dedifferentiation (< 0.001).
Loss of β -catenin membranous staining and its cytoplasmic accumulation were associated with aggressive clinicopathologic behavior. The exact effect of radioiodine exposure on β-catenin pathway remained to be determined in future.
乳头状甲状腺癌是最常见的甲状腺癌，占所有甲状腺恶性肿瘤的75%-85%。最近，β-连环蛋白已被确定在人类上皮癌的临床进程中发挥作用。本研究旨在揭示β-连环蛋白标志物与乳头状甲状腺癌行为之间的关联。
根据制造商的说明，用即用型单克隆β-连环蛋白抗体对63例乳头状甲状腺癌石蜡块进行染色。根据免疫反应强度对膜、细胞质和细胞核染色进行评分。使用SPSS 15版分析β-连环蛋白免疫染色与复发次数和放射性碘治疗累积剂量等临床参数的关联。还评估了肿瘤分期、分级、包膜侵犯、淋巴管侵犯、淋巴结受累、远处转移等组织病理学参数以及其他变量与β-连环蛋白免疫反应性的关联。
77.8%的乳头状甲状腺癌分化良好，其余分化较差。β-连环蛋白膜免疫染色的缺失与复发次数相关（P=0.023，Pearson相关系数=-0.285）。其膜染色的缺失与放射性碘的累积剂量也有类似的相关性（P=0.046，Pearson相关系数=-0.253）。膜β-连环蛋白的缺失与一些组织病理学表现显著相关，如淋巴结受累（P<0.001）、远处转移（P=0.003）和肿瘤去分化（P<0.001）。
β-连环蛋白膜染色缺失及其细胞质积聚与侵袭性临床病理行为相关。放射性碘暴露对β-连环蛋白通路的确切影响有待未来确定。
