细胞因子疗法对实验动物模型（小鼠）包虫病治疗和预防的作用
Effects of Cytokine Therapy for Treatment and Prophylaxis of Hydatidosis in Experimental Animal Model (Mice).
作者信息
Rahdar Mahmoud, Rafiei Abdollah, Valipour Norouzi Rohollah
机构信息
Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
Dept. of Parasitology, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
出版信息
Iran J Parasitol. 2018 Oct-Dec;13(4):587-593.
BACKGROUND
We aimed to use of IL-12 and IFN-γ for prevention and treatment of hydatidosis in experimental animal models.
METHODS
This experimental study was conducted in Ahvaz City, southwest of Iran in 2017. Forty female BALB/C mice with 6-8 wk old were divided into four groups (two group test and two group control). The mice were challenged with 2000 protoscolices and a combination of IL-12 (10 μg/kg) and IFN-γ (25 μg/kg) was injected intraperitoneally daily one week at the time of challenging (prevention group) and three months after challenging (treatment group). After four months, the mice were euthanized and the number, size, and weight of the cysts were measured in all studied groups. Pathologic slides were prepared from cyst wall for pathologic changes study.
RESULTS
There issignificant reduction in number, size, and weight of cysts in test groups.
CONCLUSION
Cytokine therapy is effective methods especially for prevention of cyst formation and to treat non-operable cases. Further study of combination of chemotherapy and cytokine therapy should be investigated.
背景
我们旨在利用白细胞介素-12（IL-12）和干扰素-γ（IFN-γ）在实验动物模型中预防和治疗包虫病。
方法
这项实验研究于2017年在伊朗西南部的阿瓦士市进行。将40只6 - 8周龄的雌性BALB/C小鼠分为四组（两组试验组和两组对照组）。给小鼠接种2000个原头节，并在接种时（预防组）和接种后三个月（治疗组），每周一次腹腔注射IL-12（10μg/kg）和IFN-γ（25μg/kg）的组合，持续一周。四个月后，对小鼠实施安乐死，并测量所有研究组囊肿的数量、大小和重量。从囊肿壁制备病理切片以研究病理变化。
结果
试验组囊肿的数量、大小和重量均显著减少。
结论
细胞因子疗法是有效的方法，尤其对于预防囊肿形成和治疗无法手术的病例。应进一步研究化疗与细胞因子疗法的联合应用。
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