Amri Manel, Touil-Boukoffa Chafia
University of Sciences and Technology Houari Boumediene, Faculty of Biological Science, Laboratory of Cellular and Molecular Biology, Team 'Cytokines and NO Synthases', PB 32 El-Alia, Algiers 16111, Algeria.
University of Sciences and Technology Houari Boumediene, Faculty of Biological Science, Laboratory of Cellular and Molecular Biology, Team 'Cytokines and NO Synthases', PB 32 El-Alia, Algiers 16111, Algeria.
Asian Pac J Trop Med. 2016 Aug;9(8):749-56. doi: 10.1016/j.apjtm.2016.06.013. Epub 2016 Jun 29.
To study in vitro anti-hydatic and immunomodulatory effects of ginger and [6]-gingerol as an alternative therapy for cystic echinococcosis.
Effect of a commonly used herbal product and ginger (Zingiber officinale) towards protoscoleces (PSC) and cyst wall in vitro was studied. The effect of [6]-gingerol, and the pungent constituent of ginger, was also evaluated on PSC culture. Furthermore, the activity of both extracts in association with interferon-gamma (IFN-γ) on PSC co-cultured with mononuclear cells of hydatic patients was evaluated. The nitric oxide (NO) production was measured in each co-culture.
Ginger exhibited a concentration- and time-dependent cytotoxic effect against PSC and cyst wall. Interestingly, ginger was more effective than the [6]-gingerol. Moreover, additional parasitic effect between extracts and IFN-γ are also observed in co-cultures. Furthermore, both extracts attenuated the NO production elicited by this infection or by the IFN-γ.
Ginger has an important anti-hydatic effect in vitro. This effect is amplified in the presence of IFN-γ. Moreover, this herbal product may protect against host's cell death by reducing the high levels of NO. Ginger may act, at least, through the [6]-gingerol. All our data suggest the promising use of ginger in the treatment of Echinococcus granulosus infection.
研究生姜及[6]-姜酚对泡型棘球蚴病的体外抗虫及免疫调节作用，作为一种替代治疗方法。
研究一种常用草药产品和生姜（姜科植物姜）对原头节（PSC）和囊壁的体外作用。还评估了[6]-姜酚（生姜的辛辣成分）对PSC培养物的作用。此外，评估了两种提取物与干扰素-γ（IFN-γ）联合对与泡型棘球蚴病患者单核细胞共培养的PSC的活性。在每种共培养物中测量一氧化氮（NO）的产生。
生姜对PSC和囊壁表现出浓度和时间依赖性的细胞毒性作用。有趣的是，生姜比[6]-姜酚更有效。此外，在共培养物中还观察到提取物与IFN-γ之间的额外抗寄生虫作用。此外，两种提取物均减弱了由这种感染或IFN-γ引起的NO产生。
生姜在体外具有重要的抗虫作用。在IFN-γ存在下，这种作用会增强。此外，这种草药产品可能通过降低高水平的NO来防止宿主细胞死亡。生姜至少可能通过[6]-姜酚起作用。我们所有的数据表明生姜在治疗细粒棘球绦虫感染方面具有广阔的应用前景。
