Süli Tamás, Kozoderović Gordana, Potkonjak Aleksandar, Simin Stanislav, Simin Verica, Lalošević Vesna
Dept. of Veterinary Medicine, Faculty of Agriculture, University of Novi Sad, Novi Sad, Serbia.
Prophyl Animal Health Ltd, Mohács, Hungary.
Iran J Parasitol. 2018 Oct-Dec;13(4):594-601.
is a common protist colonizing the gastrointestinal tract of humans and various animals. Pigs have been suggested to be a reservoir for human infections because of high prevalence of the parasite in these animals and the presence of zoonotic subtypes. Nevertheless, epidemiological data is often misinterpreted due to the lack of standard diagnostic procedures. This study aimed to compare the sensitivity of different diagnostic techniques in detection of sp. in pigs.
Overall, 48 individual faecal samples were collected from pigs reared in an intensive farming system (Autonomous Province of Vojvodina, Serbia) and were tested by microscopic examination of direct wet mount, in vitro cultivation in modified Jones' medium and conventional PCR for rRNA gene.
Xenic in vitro cultivation in Jones' medium showed higher sensitivity than direct wet mount when we compared it with PCR. Namely, the estimated sensitivity of direct wet mount was 46.15%, while the sensitivity of in vitro cultivation was 84.62%.
Low sensitivity of conventional parasitological compared to molecular methods is proven. Thus, reports on prevalence that rely solely on microscopy of faecal samples (unprocessed or concentrated) are probably underestimating the true prevalence of the parasite.
是一种常见的原生生物，寄生于人类和各种动物的胃肠道。由于这种寄生虫在猪等动物中的高流行率以及人畜共患亚型的存在，猪被认为是人类感染的一个储存宿主。然而，由于缺乏标准的诊断程序，流行病学数据常常被误解。本研究旨在比较不同诊断技术检测猪体内 种的敏感性。
总体而言，从集约化养殖系统（塞尔维亚伏伊伏丁那自治省）饲养的猪中收集了48份个体粪便样本，并通过直接湿涂片显微镜检查、改良琼斯培养基体外培养和rRNA基因常规PCR进行检测。
与PCR相比，琼斯培养基中的异源体外培养显示出比直接湿涂片更高的敏感性。具体而言，直接湿涂片的估计敏感性为46.15%，而体外培养的敏感性为84.62%。
与分子方法相比，传统寄生虫学方法的敏感性较低得到了证实。因此，仅依靠粪便样本（未处理或浓缩）显微镜检查的流行率报告可能低估了寄生虫的真实流行率。
