Mirghani Seyed Javad, Peeri Maghsoud, Yaghoobpour Yekani Omid, Zamani Masoud, Feizolahi Foad, Nikbin Sina, Derakhshideh Armin, Mousavi Niloufar, Khojasteh Zohreh, Nasrollahi Zeynab, Khorasani Elya, Ghodousi Johari Elham, Afshar Tayebeh, Azarbayjani Mohammad Ali
Department of Exercise Physiology, Faculty of Physical Education and Sports Science, Islamic Azad University, Central Tehran Branch, Tehran, Islamic Republic of Iran.
Department of Exercise Physiology, Islamic Azad University, Science and Research Branch, Tehran, Islamic Republic of Iran.
JMIR Res Protoc. 2019 Jan 30;8(1):e10753. doi: 10.2196/10753.
Obesity is known as one of the major causes of epidemiologic diseases worldwide; therefore, the introduction of treatment strategies by medical professionals, such as the use of various medicines and exercise programs to reduce fat or prevent obesity, is on the rise. Recently, researchers have shown special interest in assessing the effect of lipolytic adenosine and vitamin D deficiency, as well as the effect of exercise, on decreasing body fat percentage.
This study has been designed to examine the effect of adenosine and vitamin D3 injections, in conjunction with high-intensity interval training and isocaloric moderate-intensity training, on the metabolic parameters of obesity induced by a high-fat diet.
This is an experimental study using 92 Wistar rats. At 6 weeks of age, the rats' weights will be recorded, after which they will have 1 week to adapt to their new environment before being divided into 12 groups. The rats will participate in a 2-stage experimental intervention, including a 13-week fattening diet phase followed by a 12-week exercise training phase consisting of an exercise program and the injection of adenosine and vitamin D3. Groups 1 and 2 will have a normal diet, and the other groups will have a diet of 40% fat, with free access to food and water up to the second half of the second stage of the study (end of the sixth week of training). After termination of the interventions, tissue collection and molecular assessments (blood for biochemical, tissues for gene expression analyses, and anthropometrical indexes) will be performed.
The project was initiated in April 2017 and completed in December 2017. Data analysis is under way, and the first results are expected to be submitted for publication in November 2018.
We hypothesize that weight loss-induced molecular changes and upregulation will be observed in line with an increase in lipolysis and beta oxidation in muscle and fat tissue as a result of performing isocaloric training in drug-receiving rats and groups on a high-fat diet.
INTERNATIONAL REGISTERED REPORT IDENTIFIER (IRRID): RR1-10.2196/10753.
肥胖是全球流行病学疾病的主要病因之一；因此，医学专业人员采用各种治疗策略，如使用各种药物和运动计划来减少脂肪或预防肥胖的情况正在增加。最近，研究人员对评估脂解腺苷和维生素D缺乏的影响以及运动对降低体脂百分比的影响表现出特别兴趣。
本研究旨在探讨腺苷和维生素D3注射联合高强度间歇训练和等热量中等强度训练对高脂饮食诱导的肥胖代谢参数的影响。
这是一项使用92只Wistar大鼠的实验研究。在6周龄时记录大鼠体重，之后它们将有1周时间适应新环境，然后分为12组。大鼠将参与两阶段实验干预，包括为期13周的育肥饮食阶段，随后是为期12周的运动训练阶段，包括运动计划以及腺苷和维生素D3注射。第1组和第2组将采用正常饮食，其他组将采用40%脂肪的饮食，在研究第二阶段（训练第六周结束）的后半段之前可自由获取食物和水。干预结束后，将进行组织采集和分子评估（采集血液进行生化分析、采集组织进行基因表达分析以及测量人体测量指标）。
该项目于2017年4月启动，2017年12月完成。数据分析正在进行中，预计首批结果将于2018年11月提交发表。
我们假设，在接受药物治疗的大鼠和高脂饮食组中，由于进行等热量训练，肌肉和脂肪组织中的脂解和β氧化增加，将观察到体重减轻引起的分子变化和上调。
国际注册报告识别码（IRRID）：RR1-10.2196/10753。
