Holyavka M G, Artyukhov V G, Sazykina S M
Radiats Biol Radioecol. 2017 Jan;57(1):66-70.
We investigated the mechanism of UV-radiation influence on trypsin in free and immobilized (on chitosan) states. The catalytic activity of free enzyme under the action of UV-light is subjected to changes to a greater extent than that of the immobilized one. We assume that the photoprotection effect of chitosan is caused for the following reasons: firstly, through interactaction with trypsin molecules chitosan forms a more photoresistant complex as compared to the native protein; secondly, chitosan probably binds the active photopro- ducts of a free radical nature, thus preventing oxidation (destruction) of several amino acids of the enzyme under its UV-radiation.
我们研究了紫外线辐射对游离态和固定化（壳聚糖固定）态胰蛋白酶的影响机制。与固定化酶相比，游离酶在紫外线作用下的催化活性变化程度更大。我们认为壳聚糖的光保护作用是由以下原因引起的：首先，壳聚糖与胰蛋白酶分子相互作用，形成了比天然蛋白质更耐光的复合物；其次，壳聚糖可能结合了自由基性质的活性光产物，从而防止酶在紫外线辐射下的几个氨基酸发生氧化（破坏）。
