Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, Sichuan, China.
Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, Sichuan, China.
PLoS One. 2019 Jan 30;14(1):e0209570. doi: 10.1371/journal.pone.0209570. eCollection 2019.
Health benefits, flavour quality indicators and physical properties were analysed after feeding grass carp graded concentrations of soybean isoflavones (SIF) (0, 25, 50, 75, 100 and 125 mg/kg) for 60 days. The results demonstrated that optimal dietary SIF supplementation improved the protein and total PUFA content, especially healthcare n-3 PUFA (C18: 3n-3, EPA and DHA), and increased the flavour-related free amino acid [especially umami amino acid] and 5'-inosine monophosphate content, improving the health benefits and flavour quality indicators in the muscle of grass carp. In addition, optimal dietary SIF supplementation (25 or 50 mg SIF/kg diet) enhanced some physical properties [water-holding capacity and tenderness] and increased the collagen content; however, it reduced cathepsin activity and apoptosis. SIF supplementation enhanced the glutathione content and the activity of antioxidant enzymes (except CuZnSOD) by regulating their gene expression. The gene expression could be regulated by NF-E2-related factor 2 (Nrf2) signalling in the muscle of grass carp. We demonstrated that optimal dietary SIF supplementation elevated the health benefits, flavour quality indicators and physical properties of fish muscle.
在 60 天的时间里，给草鱼投喂不同梯度浓度的大豆异黄酮（SIF）（0、25、50、75、100 和 125mg/kg）后，分析了其对健康的益处、风味品质指标和物理特性。结果表明，最佳饮食 SIF 补充可提高蛋白质和总多不饱和脂肪酸（PUFA）含量，特别是保健 n-3 PUFA（C18:3n-3、EPA 和 DHA），并增加与风味相关的游离氨基酸[特别是鲜味氨基酸]和 5'-肌苷单磷酸的含量，从而提高草鱼肌肉的健康益处和风味品质指标。此外，最佳饮食 SIF 补充（25 或 50mg SIF/kg 饲料）可增强一些物理特性[持水能力和嫩度]，增加胶原蛋白含量；然而，它降低了组织蛋白酶活性和细胞凋亡。SIF 补充通过调节其基因表达，增加了谷胱甘肽含量和抗氧化酶（CuZnSOD 除外）的活性。基因表达可以通过草鱼肌肉中的 NF-E2 相关因子 2（Nrf2）信号通路进行调节。我们证明了最佳饮食 SIF 补充可提高鱼肉的健康益处、风味品质指标和物理特性。
