Laboratory of Materials Science, Instituto de Quimica de Recursos Naturales, Universidad de Talca, 747 Talca, Chile.
Centre de Investigacion de Polimeros Avanzados, CIPA, avenida Collao 1202, Edificio de Laoratorios, Concepcion, Chile.
Int J Biol Macromol. 2019 May 1;128:499-508. doi: 10.1016/j.ijbiomac.2019.01.145. Epub 2019 Jan 27.
Chitosan (CH) capped inorganic nanomaterials have been considered as significant antibacterial materials in the clinical field. This work shows the synthesis of two new different antibacterial composite films as a result of the incorporation of CH capped copper oxide (CHCuO) and copper (CHCu) nanoparticles (NPs). Here, CHCuO and CHCu NPs were achieved by a facile chemical reduction of Cu ions using sodium hydroxide and ascorbic acid. TEM analysis revealed the morphology as rod-type nanoflakes for CHCuO and a spherical shape for CHCu NPs with ~7 ± 2 nm size. Antimicrobial activity of the developed materials was studied by the inhibition zone method, against both gram-negative and gram-positive bacteria. The antimicrobial activity revealed that the CHCuO NPs and CHCuO-CH film showed a higher inhibition zone than the other nanomaterials. The results suggested that the synthesized materials can be used in wound dressing applications.
壳聚糖（CH）包覆的无机纳米材料已被认为是临床领域有重要意义的抗菌材料。本工作展示了两种新型抗菌复合膜的合成，其是通过将 CH 包覆的氧化铜（CHCuO）和铜（CHCu）纳米粒子（NPs）掺入而得到的。在这里，CHCuO 和 CHCu NPs 是通过使用氢氧化钠和抗坏血酸还原 Cu 离子而轻易地实现的。TEM 分析表明，CHCuO 具有棒状纳米薄片形态，而 CHCu NPs 具有 ~7 ± 2 nm 大小的球形形态。通过抑菌圈法研究了所开发材料对革兰氏阴性菌和革兰氏阳性菌的抗菌活性。抗菌活性表明，CHCuO NPs 和 CHCuO-CH 薄膜比其他纳米材料具有更高的抑菌圈。结果表明，所合成的材料可用于伤口敷贴应用。
