Department of Chemistry, Amirkabir University of Technology (Polytechnic), P.O. Box: 15875-4413, Tehran, Iran.
Int J Biol Macromol. 2013 Sep;60:226-34. doi: 10.1016/j.ijbiomac.2013.05.026. Epub 2013 Jun 5.
New nanocomposite films of chitosan/phosphoramide/1-5% Fe3O4 NPs were prepared. The Fe3O4 NPs were synthesized as core-shell NPs with starch as a capping agent. The phosphoramide compound was synthesized from the reaction of morpholine with N-4-nitrophenyl phosphoramidic dichloride. The XRD and SEM analysis of Fe3O4 NPs, chitosan (CS), phosphoramide (Ph), CS/Ph and CS/Fe3O4 NPs films as well as the nanocomposite films 1-5 containing CS/Ph/1-5% Fe3O4 NPs were investigated and the results confirmed the formation of the desired films. The in vitro antibacterial activities were evaluated against four bacteria including two Gram-positive Staphylococcus aureus (S. aureus), Bacillus cereus (B. cereus) and two Gram-negative Escherchia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa) bacteria. Results indicated greater antibacterial effects for the films against Gram-positive bacteria. In addition, nanocomposite films containing higher percent of Fe3O4 NPs displayed more antibacterial activities.
制备了壳聚糖/磷酰胺/1-5%Fe3O4 NPs 的新型纳米复合膜。Fe3O4 NPs 是通过用淀粉作为封端剂合成核壳 NPs 而得到的。磷酰胺化合物是由吗啉与 N-4-硝基苯磷酰胺二氯反应合成的。对 Fe3O4 NPs、壳聚糖 (CS)、磷酰胺 (Ph)、CS/Ph 和 CS/Fe3O4 NPs 薄膜以及含有 CS/Ph/1-5%Fe3O4 NPs 的纳米复合薄膜 1-5 进行了 XRD 和 SEM 分析，结果证实了所需薄膜的形成。对四种细菌（包括两种革兰氏阳性菌金黄色葡萄球菌（S. aureus）、蜡状芽孢杆菌（B. cereus）和两种革兰氏阴性菌大肠杆菌（E. coli）、铜绿假单胞菌（P. aeruginosa））进行了体外抗菌活性评估。结果表明，薄膜对革兰氏阳性菌具有更强的抗菌作用。此外，含有更高百分比 Fe3O4 NPs 的纳米复合膜显示出更强的抗菌活性。
