Caffier Valérie, Patocchi Andrea, Expert Pascale, Bellanger Marie-Noëlle, Durel Charles-Eric, Hilber-Bodmer Maja, Broggini Giovanni A L, Groenwold Remmelt, Bus Vincent G M
INRA, UMR1345 Institut de Recherche en Horticulture et Semences, 49071 Beaucouzé, France; AgroCampus-Ouest, UMR1345 Institut de Recherche en Horticulture et Semences, 49045 Angers, France; Université d'Angers, UMR1345 Institut de Recherche en Horticulture et Semences, SFR 4207 QUASAV, 49045 Angers, France.
Agroscope Research Station, Phytopathology, P.B., 8820 Wädenswil, Switzerland.
Plant Dis. 2015 Mar;99(3):370-375. doi: 10.1094/PDIS-07-14-0708-RE.
A set of differential hosts has recently been identified for 17 apple scab resistance genes in an updated system for defining gene-for-gene (GfG) relationships in the Venturia inaequalis-Malus pathosystem. However, a set of reference isolates characterized for their complementary avirulence alleles is not yet available. In this paper, we report on improving the set of differential hosts for h(7) and propose the apple genotype LPG3-29 as carrying the single major resistance gene Rvi7. We characterized a reference set of 23 V. inaequalis isolates on 14 differential apple hosts carrying major resistance genes under controlled conditions. We identified isolates that were virulent on at least one of the following defined resistance gene hosts: h(1), h(2), h(3), h(4), h(5), h(6), h(7), h(8), h(9), h(10), and h(13). Sixteen different virulence patterns were observed. In general, the isolates carried one to three virulences, but some of them were more complex, with up to six virulences. This set of well-characterized isolates will be helpful for the identification of additional apple scab resistance genes in apple germplasm and the characterization of new GfG relationships to help improve our understanding of the host-pathogen interactions in the V. inaequalis-Malus pathosystem.
在一个用于定义苹果黑星病菌-苹果病理系统中基因对基因（GfG）关系的更新系统中，最近已为17个苹果黑星病抗性基因鉴定出一组鉴别寄主。然而，一组以其互补无毒等位基因为特征的参考分离株尚不可用。在本文中，我们报告了改进h(7)鉴别寄主集的情况，并提出苹果基因型LPG3-29携带单一主要抗性基因Rvi7。我们在可控条件下，对携带主要抗性基因的14个鉴别苹果寄主上的23个苹果黑星病菌分离株参考集进行了特征分析。我们鉴定出了在以下至少一种已定义抗性基因寄主上具有毒性的分离株：h(1)、h(2)、h(3)、h(4)、h(5)、h(6)、h(7)、h(8)、h(9)、h(10)和h(13)。观察到16种不同的毒性模式。一般来说，分离株具有一到三种毒性，但其中一些更复杂，具有多达六种毒性。这组特征明确的分离株将有助于在苹果种质中鉴定额外的苹果黑星病抗性基因，以及鉴定新的GfG关系，以帮助我们更好地理解苹果黑星病菌-苹果病理系统中的寄主-病原体相互作用。
