Peil Andreas, Howard Nicholas P, Bühlmann-Schütz Simone, Hiller Ines, Schouten Henk, Flachowsky Henryk, Patocchi Andrea
Julius Kühn Institut (JKI)-Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Pillnitzer Platz 3a, 01326 Dresden, Pillnitz Germany.
Fresh Forward Breeding and Marketing B.V., Hogewoerd 1C, 6851 ET Huissen, The Netherlands.
Mol Breed. 2023 Oct 11;43(10):74. doi: 10.1007/s11032-023-01421-0. eCollection 2023 Oct.
The apple ( x ) scab () resistance genes and were mapped to a similar region on the top of linkage group 2 and both resistance genes elicit the same type of resistance reaction, i.e., a hypersensitive response; hence, it is suspected that the two genes may be the same. As the two resistance genes and are currently used in apple breeding, it is important to clarify whether the two resistance genes are the same or not. Several approaches were used to make this determination. First, the pedigree of the genotype GMAL 2473, the source of , was reconstructed. GMAL 2473 was found to be an F1 of 'Russian seedling', the genotype, which is known to also be the source of . Next, it was further demonstrated that 'Regia', a cultivar known to carry (and ), carries the same gene (), which was demonstrated to be the gene inducing resistance. Finally, it was shown that transgenic lines carrying are compatible with race apple scab isolates. Taken all together, these results definitively demonstrate that and are the same resistance gene. For future studies, we suggest referring to this resistance with the first name that was assigned to this gene, namely .
The online version contains supplementary material available at 10.1007/s11032-023-01421-0.
苹果黑星病抗性基因 和 被定位到连锁群2顶部的相似区域，且这两个抗性基因引发相同类型的抗性反应，即过敏反应；因此，怀疑这两个基因可能相同。由于这两个抗性基因 和 目前用于苹果育种，明确这两个抗性基因是否相同很重要。采用了几种方法来进行此判定。首先，重建了 的来源基因型GMAL 2473的系谱。发现GMAL 2473是‘俄罗斯实生苗’的F1代，已知该基因型也是 的来源。接下来，进一步证明，已知携带 （和 ）的品种‘雷吉亚’携带相同的基因（ ），已证明该基因诱导 抗性。最后，表明携带 的转基因系与苹果黑星病菌株小种兼容。综合来看，这些结果明确证明 和 是相同的抗性基因。对于未来的研究，我们建议用首次赋予该基因的名称，即 来指代这种抗性。
在线版本包含可在10.1007/s11032-023-01421-0获取的补充材料。
