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并且 是相同的苹果黑星病抗性基因。 需注意,原英文句子表述不太完整规范,翻译出来的中文也稍显生硬,但严格按照要求进行了翻译。

and are the same apple scab resistance genes.

作者信息

Peil Andreas, Howard Nicholas P, Bühlmann-Schütz Simone, Hiller Ines, Schouten Henk, Flachowsky Henryk, Patocchi Andrea

机构信息

Julius Kühn Institut (JKI)-Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Fruit Crops, Pillnitzer Platz 3a, 01326 Dresden, Pillnitz Germany.

Fresh Forward Breeding and Marketing B.V., Hogewoerd 1C, 6851 ET Huissen, The Netherlands.

出版信息

Mol Breed. 2023 Oct 11;43(10):74. doi: 10.1007/s11032-023-01421-0. eCollection 2023 Oct.


DOI:10.1007/s11032-023-01421-0
PMID:37830083
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10564682/
Abstract

UNLABELLED: The apple ( x ) scab () resistance genes and were mapped to a similar region on the top of linkage group 2 and both resistance genes elicit the same type of resistance reaction, i.e., a hypersensitive response; hence, it is suspected that the two genes may be the same. As the two resistance genes and are currently used in apple breeding, it is important to clarify whether the two resistance genes are the same or not. Several approaches were used to make this determination. First, the pedigree of the genotype GMAL 2473, the source of , was reconstructed. GMAL 2473 was found to be an F1 of 'Russian seedling', the genotype, which is known to also be the source of . Next, it was further demonstrated that 'Regia', a cultivar known to carry (and ), carries the same gene (), which was demonstrated to be the gene inducing resistance. Finally, it was shown that transgenic lines carrying are compatible with race apple scab isolates. Taken all together, these results definitively demonstrate that and are the same resistance gene. For future studies, we suggest referring to this resistance with the first name that was assigned to this gene, namely . SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11032-023-01421-0.

摘要

未标注:苹果黑星病抗性基因 和 被定位到连锁群2顶部的相似区域,且这两个抗性基因引发相同类型的抗性反应,即过敏反应;因此,怀疑这两个基因可能相同。由于这两个抗性基因 和 目前用于苹果育种,明确这两个抗性基因是否相同很重要。采用了几种方法来进行此判定。首先,重建了 的来源基因型GMAL 2473的系谱。发现GMAL 2473是‘俄罗斯实生苗’的F1代,已知该基因型也是 的来源。接下来,进一步证明,已知携带 (和 )的品种‘雷吉亚’携带相同的基因( ),已证明该基因诱导 抗性。最后,表明携带 的转基因系与苹果黑星病菌株小种兼容。综合来看,这些结果明确证明 和 是相同的抗性基因。对于未来的研究,我们建议用首次赋予该基因的名称,即 来指代这种抗性。 补充信息:在线版本包含可在10.1007/s11032-023-01421-0获取的补充材料。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0a/10564682/70b44b9ba1bd/11032_2023_1421_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0a/10564682/447bb4ab3006/11032_2023_1421_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0a/10564682/70b44b9ba1bd/11032_2023_1421_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0a/10564682/447bb4ab3006/11032_2023_1421_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dc0a/10564682/70b44b9ba1bd/11032_2023_1421_Fig2_HTML.jpg

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[3]
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[4]
A new method to reconstruct the direction of parent-offspring duo relationships using SNP array data and its demonstration on ancient and modern cultivars in the outcrossing species malus × domestica.

Hortic Res. 2022-1-19

[5]
Ten Years of VINQUEST: First Insight for Breeding New Apple Cultivars With Durable Apple Scab Resistance.

Plant Dis. 2020-6-11

[6]
New North American Isolates of Can Overcome Apple Scab Resistance of 821.

Plant Dis. 2020-1-21

[7]
High-quality, genome-wide SNP genotypic data for pedigreed germplasm of the diploid outbreeding species apple, peach, and sweet cherry through a common workflow.

PLoS One. 2019-6-27

[8]
A high-quality apple genome assembly reveals the association of a retrotransposon and red fruit colour.

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[9]
Virulence Characterization of Venturia inaequalis Reference Isolates on the Differential Set of Malus Hosts.

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[10]
An integrated approach for increasing breeding efficiency in apple and peach in Europe.

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