a Department of Chemical Engineering and Biotechnological Engineering , Faculty of Engineering, University of Sherbrooke , Sherbrooke , Canada.
Crit Rev Biotechnol. 2019 May;39(3):337-350. doi: 10.1080/07388551.2019.1566202. Epub 2019 Jan 30.
Thermoduric pullulanases, acting as starch-debranching enzymes, are required in many industrial applications, mainly in the production of concentrated glucose, maltose, and fructose syrups. To date, however, a single pullulanase, from Bacillus acidopullulyticus, is available on the market for industrial purposes. This review is an investigation of the major advances as well as the major challenges being faced with regard to optimization of the production of extracellular thermoduric pullulanases either by their original hosts or by recombinant organisms. The critical aspects linked to industrial pullulanase production, which should always be considered, are emphasized, including those parameters influencing solubility, thermostability, and catalytic efficiency of the enzyme. This review provides new insights for improving the production of extracellular thermoduric pullulanases in the hope that such information may facilitate their commercial utilization and potentially be applied to the development of other industrially relevant enzymes.
耐热 pullulanases，作为淀粉分支酶，在许多工业应用中是必需的，主要用于生产浓缩葡萄糖、麦芽糖和果糖糖浆。然而，迄今为止，市场上仅有一种 pullulanase，来自解淀粉芽孢杆菌，可用于工业用途。本综述调查了在通过原始宿主或重组生物优化胞外耐热 pullulanase 的生产方面取得的主要进展和面临的主要挑战。强调了与工业 pullulanase 生产相关的关键方面，包括影响酶的溶解度、热稳定性和催化效率的参数。本综述为提高胞外耐热 pullulanase 的生产提供了新的见解，希望这些信息可以促进它们的商业利用，并可能应用于开发其他工业相关的酶。
