Naik Bindu, Kumar Vijay, Goyal S K, Dutt Tripathi Abhishek, Mishra Sadhna, Joakim Saris Per Erik, Kumar Akhilesh, Rizwanuddin Sheikh, Kumar Vivek, Rustagi Sarvesh
Department of Food Science and Technology, Graphic Era (Deemed to be University), Uttarakhand, India.
Himalayan School of Biosciences, Swami Rama Himalayan University, Dehradun, India.
Front Bioeng Biotechnol. 2023 Jun 28;11:1139611. doi: 10.3389/fbioe.2023.1139611. eCollection 2023.
Pullulanases are the most important industrial group of enzymes in family 13 glycosyl hydrolases. They hydrolyze either α-1,6 and α-1,4 or both glycosidic bonds in pullulan as well as other carbohydrates to produce glucose, maltose, and maltotriose syrups, which have important uses in food and other related sectors. However, very less reports are available on pullulanase production from native strains because of low yield issues. In line with the increasing demands for pullulanase, it has become important to search for novel pullulanase-producing microorganisms with high yields. Moreover, high production costs and low yield are major limitations in the industrial production of pullulanase enzymes. The production cost of pullulanase by using the solid-state fermentation (SSF) process can be minimized by selecting agro-industrial waste. This review summarizes the types, sources, production strategies, and potential applications of pullulanase in different food and other related industries. Researchers should focus on fungal strains producing pullulanase for better yield and low production costs by using agro-waste. It will prove a better enzyme in different food processing industries and will surely reduce the cost of products.
普鲁兰酶是13家族糖基水解酶中最重要的工业酶类。它们能水解支链淀粉以及其他碳水化合物中的α-1,6和α-1,4糖苷键或两者，以产生葡萄糖、麦芽糖和麦芽三糖糖浆，这些糖浆在食品及其他相关领域有重要用途。然而，由于产量低的问题，关于从天然菌株生产普鲁兰酶的报道非常少。随着对普鲁兰酶需求的增加，寻找高产的新型普鲁兰酶生产微生物变得很重要。此外，高生产成本和低产量是普鲁兰酶工业生产的主要限制因素。通过选择农业工业废弃物，采用固态发酵（SSF）工艺生产普鲁兰酶的成本可以降至最低。本综述总结了普鲁兰酶在不同食品及其他相关行业中的类型、来源、生产策略和潜在应用。研究人员应专注于利用农业废弃物生产普鲁兰酶的真菌菌株，以获得更好的产量和更低的生产成本。它将在不同的食品加工业中成为一种更好的酶，并肯定会降低产品成本。
