Technology Joint Management Office, Research Promotion Division, Tokai University.
Department of Applied Biochemistry, Tokai University.
Biosci Trends. 2019 Mar 14;13(1):86-90. doi: 10.5582/bst.2019.01008. Epub 2019 Jan 30.
The current study determined the structure of a hemolytic compound found in an extract from the fruiting bodies of the edible mushroom Hypsizygus marmoreus when its pH was lowered. The hemolytic compound was purified using the modified Bligh and Dyer method followed by chromatography using reversed phase and silica gel columns. Structural analyses of the purified hemolytic compound were performed using NMR and ESI-MS. The deduced structure indicated a trans,trans-5,8-docosadienoic acid calcium salt. Although numerous proteinous hemolysins from various mushrooms have been described, the current study is the first to report on a low-molecular-weight hemolytic compound derived from an H. marmoreus extract.
本研究在降低 pH 值时，从可食用蘑菇大理石蕈的子实体提取物中确定了一种溶血化合物的结构。溶血化合物使用改良的 Bligh 和 Dyer 法提取，然后使用反相和硅胶柱层析进行纯化。使用 NMR 和 ESI-MS 对纯化的溶血化合物进行结构分析。推断的结构表明为反式，反式-5,8-二十二碳二烯酸钙盐。尽管已经描述了来自各种蘑菇的许多蛋白质溶血素，但本研究是首次报道源自大理石蕈提取物的低分子量溶血化合物。
