Engineering Research Center in Biomaterials, Sichuan University, Chengdu 610064, China.
National Engineering Research Center for Biomaterials, Chengdu 610064, China.
J Biomed Mater Res A. 2019 Jun;107(6):1253-1263. doi: 10.1002/jbm.a.36634. Epub 2019 Feb 15.
Oxidative stress could cause damage to lipids, proteins and DNA, which is induced by the imbalance between the production of reactive oxygen species (ROS) and the biological system ability to counteract or detoxify their harmful effects. The oxidative stress damage significantly contributes to a number of diseases. Magnesium (Mg) is endowed with a novel function of removing excess ROS by releasing H during the degradation. In this study, in order to explore the property of anti-oxidative damage of Mg metal, rat bone marrow mesenchymal stem cells (MSCs) oxidative damaged by ultraviolet (UV) radiation was employed to co-culture with Mg metal. The effect of Mg metal on the response of antioxidant enzymes and mitochondria in MSCs was studied. We found that Mg metal could reduce the cellular oxidative stress damage and elevate the activities of antioxidant enzymes to maintain redox homeostasis. In addition, Mg metal could reduce the risk of UV-induced cell apoptosis by increasing the ratio of Bcl-2/Bax, elevating the mitochondrial membrane potential and blocking the release of cytochrome c. This finding showed Mg metal might have the potential for treating diseases caused by oxidative stress damage. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 1253-1263, 2019.
氧化应激会导致脂质、蛋白质和 DNA 损伤，这是由活性氧（ROS）的产生与生物系统清除或解毒其有害影响的能力之间的失衡引起的。氧化应激损伤是许多疾病的重要诱因。镁（Mg）在降解过程中通过释放 H+来清除多余的 ROS，从而具有新的功能。在这项研究中，为了探索 Mg 金属的抗氧化损伤特性，采用紫外线（UV）辐射诱导的大鼠骨髓间充质干细胞（MSCs）氧化损伤模型与 Mg 金属共培养。研究了 Mg 金属对 MSCs 抗氧化酶和线粒体反应的影响。结果发现，Mg 金属可以减轻细胞氧化应激损伤，提高抗氧化酶的活性，维持氧化还原平衡。此外，Mg 金属可以通过增加 Bcl-2/Bax 的比值、提高线粒体膜电位和阻止细胞色素 c 的释放，降低 UV 诱导的细胞凋亡的风险。这一发现表明，Mg 金属可能具有治疗氧化应激损伤相关疾病的潜力。© 2019 Wiley Periodicals, Inc. J 生物材料研究杂志 A 部分：107A:1253-1263, 2019.
