Lankin V Z, Tikhaze A K, Konovalova G G, Odinokova O A, Doroshchuk N A, Chazova I E
National Medical Research Center for Cardiology, Moscow, Russia.
I.M. Sechenov First Moscow State Medical University, Ministry of Health of Russia (Sechenov University), Moscow, Russia.
Ter Arkh. 2018 Nov 22;90(10):46-50. doi: 10.26442/terarkh2018901046-50.
To study the oxidative damage of biopolymers (proteins and nucleic acids) in blood of patients with type 2 diabetes mellitus (DM).
In the blood of 50 patients with DM and 25 patients without disorders of carbohydrate metabolism were estimated: the level of oxidized low-density lipoprotein (oxLDL) by immunochemical method, the content of SH-groups in plasma proteins, the activity of Cu, Zn-superoxide dismutase (SOD) in erythrocytes, the length of telomere in leukocyte DNA, the level of 8-hydroxy-2'-deoxygunosine (8-oxo-dG) in plasma and urine.
It is shown that in DM patients the level of oxLDL increases and the content of SH-groups in proteins and peptides of the blood plasma decreases, which indicates the development of oxidative stress. In addition, a carbonyl-dependent modification of erythrocyte SOD was detected in DM patients, as well as oxidative DNA destruction (decrease in telomere length in leukocytes and an increase in the level of 8-oxo-dG in blood plasma and urine).
On the basis of the definition of a complex of correct indicators, a multiple oxidative modification of biopolymers of blood (proteins and DNA) was detected in patients with DM.
研究2型糖尿病（DM）患者血液中生物聚合物（蛋白质和核酸）的氧化损伤情况。
对50例糖尿病患者和25例无碳水化合物代谢紊乱患者的血液进行检测：采用免疫化学方法测定氧化型低密度脂蛋白（oxLDL）水平、血浆蛋白中SH基团含量、红细胞中铜锌超氧化物歧化酶（SOD）活性、白细胞DNA中端粒长度、血浆和尿液中8-羟基-2'-脱氧鸟苷（8-氧代-dG）水平。
结果显示，糖尿病患者中oxLDL水平升高，血浆中蛋白质和肽段的SH基团含量降低，这表明氧化应激的发生。此外，在糖尿病患者中检测到红细胞SOD的羰基依赖性修饰，以及氧化性DNA损伤（白细胞端粒长度缩短，血浆和尿液中8-氧代-dG水平升高）。
基于一系列正确指标的确定，在糖尿病患者中检测到血液生物聚合物（蛋白质和DNA）的多重氧化修饰。
