Gruzdeva O V, Borodkina A D, Akbasheva O E, Dileva Yu A, Antonova L V, Matveeva V G, Uchasova E G, Ivanov S V, Belik E V, Fanaskova E V, Karetnikova V N, Kokov A N, Barbarash O L
Research Institute for Complex Issues of Cardiovascular Disease, Kemerovo, Russia.
Siberian State Medical University, Ministry of Health of Russia, Tomsk, Russia.
Ter Arkh. 2018 Nov 22;90(10):71-78. doi: 10.26442/terarkh2018901071-78.
To study adipokine-cytokine profile of epicardial adipocytes (EAT) and subcutaneous adipose tissue (SAT) in conjunction with the area of visceral adipose tissue (VAT), biochemical and clinical characteristics of patients with coronary heart disease.
Examined 84 patients (70 men and 14 women) with coronary artery disease. In fact the presence of visceral obesity (VO) the patients were divided into two groups. Patients VO the sampling of adipocytes of EAT and SAT, with subsequent cultivation and evaluation of adipokine and provospalitelna activity. Carried out the determination of carbohydrate and lipid metabolism, adipokine and pro-inflammatory status in the blood serum.
It was found that adipokine-cytokine profile of adipocytes of EAT and SAT differ. Adipocytes art of the disease on the background characterized by an increase IL-1, TNF-α, leptin-adiponectin relationships and a decrease in the content of protective factors: adiponectin and anti-inflammatory cytokine IL-10. While the SAT adipocytes was characterized by a decrease in the concentration of soluble receptor for leptin and the more pronounced leptinresistance, and the increase in proinflammatory cytokines was offset by the increase in the concentration of IL-10. The presence associated with multi-vessel coronary bed lesion, multifocal atherosclerosis, insulin resistance, atherogenic dyslipidemia, an imbalance of adipokines and markers of inflammation. So the value of the square VAT determined higher concentrations of leptin, TNF-α in adipocytes and serum, lipid and carbohydrate metabolism and a lower content of soluble receptor for leptin.
Thus, the disease on the background of the status of the adipocytes of EAT characterized as a "metabolic inflammation", and may indicate the direct involvement of adipocytes in the pathogenesis of coronary artery disease, due to the formation of adipokine imbalance and the activation of proinflammatory reactions.
研究冠心病患者的心外膜脂肪细胞（EAT）和皮下脂肪组织（SAT）的脂肪因子 - 细胞因子谱，以及与内脏脂肪组织（VAT）面积、生化和临床特征的关系。
检查了84例冠心病患者（70例男性和14例女性）。根据是否存在内脏肥胖（VO）将患者分为两组。对VO患者采集EAT和SAT的脂肪细胞，随后进行培养并评估脂肪因子和促炎活性。测定血清中的碳水化合物和脂质代谢、脂肪因子和促炎状态。
发现EAT和SAT的脂肪细胞的脂肪因子 - 细胞因子谱不同。疾病背景下的EAT脂肪细胞其特征为白细胞介素 - 1（IL - 1）、肿瘤坏死因子 - α（TNF - α）、瘦素 - 脂联素比值增加，以及保护因子脂联素和抗炎细胞因子IL - 10的含量降低。而SAT脂肪细胞的特征是瘦素可溶性受体浓度降低和更明显的瘦素抵抗，并且促炎细胞因子的增加被IL - 10浓度的增加所抵消。存在与多支冠状动脉病变、多灶性动脉粥样硬化、胰岛素抵抗、致动脉粥样硬化血脂异常、脂肪因子失衡和炎症标志物相关。因此，VAT面积的值与脂肪细胞和血清中更高浓度的瘦素、TNF - α、脂质和碳水化合物代谢以及更低含量的瘦素可溶性受体相关。
因此，EAT脂肪细胞状态背景下的疾病特征为“代谢性炎症”，并且可能表明脂肪细胞直接参与冠状动脉疾病的发病机制，这是由于脂肪因子失衡的形成和促炎反应的激活。
