MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering , Zhejiang University , Hangzhou 310027 , China.
ACS Appl Mater Interfaces. 2019 Feb 20;11(7):6789-6795. doi: 10.1021/acsami.8b20069. Epub 2019 Feb 12.
Enzymatic cascade reactions in confined microenvironments play important roles in cellular chemical transformation. They also have important biotechnological and therapeutic applications. Here, enzymatic cascade microreactors (MRs) coupling glucose oxidase (GOx) and hemoglobin (Hb) (GOx-Hb MRs) were successfully fabricated by co-precipitation of GOx and Hb into a MnCO template, followed by the assembly of a multilayer film on a template surface, slight cross-linking, and final removal of MnCO. In the presence of glucose with blood-relevant concentration, the GOx-Hb MRs exhibited a higher cascade reaction activity under mild acidic conditions than that under neutral conditions at physiological temperature. The GOx-Hb MRs effectively consumed glucose to generate HO at pH = 5, which significantly inhibited bacterial growth and biofilm formation. This kind of enzymatic cascade microreactors might be useful for applications in biomedical fields.
在受限的微环境中，酶级联反应在细胞化学转化中起着重要作用。它们也有重要的生物技术和治疗应用。在这里，通过将葡萄糖氧化酶 (GOx) 和血红蛋白 (Hb) 共沉淀到 MnCO 模板中，然后在模板表面组装多层膜、轻微交联，最后去除 MnCO，成功制备了酶级联微反应器 (MRs) (GOx-Hb MRs)。在具有血液相关浓度的葡萄糖存在下，GOx-Hb MRs 在生理温度下的温和酸性条件下比中性条件下表现出更高的级联反应活性。GOx-Hb MRs 有效地消耗葡萄糖以在 pH = 5 时生成 HO，这显著抑制了细菌的生长和生物膜的形成。这种酶级联微反应器在生物医学领域的应用可能是有用的。
