Max Planck Institute for Polymer Research, Ackermannweg 10, Mainz, 55128, Germany.
School of Chemical Engineering & Materials Science, Chung-Ang University, Heukseok-ro 84 Dongjak-gu, Seoul, 06974, Republic of Korea.
Adv Sci (Weinh). 2022 Apr;9(10):e2104884. doi: 10.1002/advs.202104884. Epub 2021 Dec 22.
Colloidal assemblies of mesoporous suprastructures provide effective catalysis in an advantageous volume-confined environment. However, typical fabrication methods of colloidal suprastructures are carried out under toxic or harmful conditions for unstable biomolecules, such as, biocatalytic enzymes. For this reason, biocatalytic enzymes have rarely been used with suprastructures, even though biocatalytic cascade reactions in confined environments are more efficient than in open conditions. Here, multimodal enzyme- and photocatalyst-carrying superstructures with efficient cascade reactions for colorimetric glucose detection are demonstrated. The suprastructures consisting of various functional nanoparticles, including enzyme-carrying nanoparticles, are fabricated by surface-templated evaporation driven suprastructure synthesis on polydimethylsiloxane-grafted surfaces at ambient conditions. For the fabrication of suprastructures, no additional chemicals and reactions are required, which allows maintaining the enzyme activities. The multimodal enzymes (glucose oxidase and peroxidase)-carrying suprastructures exhibit rapid and highly sensitive glucose detection via two enzyme cascade reactions in confined geometry. Moreover, the combination of enzymatic and photocatalytic cascade reactions of glucose oxidase to titanium dioxide nanoparticles is successfully realized for the same assay. These results show promising abilities of multiple colloidal mixtures carrying suprastructures for effective enzymatic reactions and open a new door for advanced biological reactions and enzyme-related works.
介孔超结构的胶体组装体在有利的体积受限环境中提供有效的催化作用。然而，胶体超结构的典型制造方法是在对不稳定生物分子（如生物催化酶）有毒或有害的条件下进行的。由于这个原因，生物催化酶很少与超结构一起使用，尽管在封闭环境中的生物催化级联反应比在开放条件下更有效。在这里，展示了具有高效级联反应的多模式酶和光催化剂负载超结构，用于比色葡萄糖检测。由包括酶负载纳米颗粒在内的各种功能纳米颗粒组成的超结构是通过在环境条件下在聚二甲基硅氧烷接枝表面上进行的表面模板蒸发驱动的超结构合成来制造的。对于超结构的制造，不需要额外的化学物质和反应，这允许保持酶的活性。多模式酶（葡萄糖氧化酶和过氧化物酶）负载的超结构通过在受限几何形状中的两种酶级联反应表现出快速和高度敏感的葡萄糖检测。此外，葡萄糖氧化酶到二氧化钛纳米颗粒的酶促和光催化级联反应的组合也成功地用于相同的测定。这些结果表明，携带超结构的多种胶体混合物具有进行有效酶反应的有前途的能力，并为先进的生物反应和与酶相关的工作开辟了新的途径。
