Tomas J M, Camprubi S, Williams P
Department of Microbiology, Faculty of Biology, University of Barcelona, Spain.
Microb Pathog. 1988 Aug;5(2):141-7. doi: 10.1016/0882-4010(88)90016-2.
Isogenic non-encapsulated (K-) mutants (O1:K-) were obtained from several different Klebsiella pneumoniae O1:K1 serotype strains. By employing K. pneumoniae bacteriophages FC3-1, FC3-2, and bacteriophage phi 1, the bacterial surface receptors of which, are the O1-antigen of lipopolysaccharide and the K1 capsular polysaccharide (K1) respectively, the K1 polysaccharide was found to completely cover the O1 lipopolysaccharide molecules in each of the O1:K1 strains examined. Exposure of the O-antigen at the cell surface was only observed after growth in the presence of sub-minimum inhibitory concentrations of antibiotics. The implications of these findings for the design of vaccines for the prevention of Klebsiella infections is discussed.
同基因非包膜（K-）突变体（O1:K-）是从几种不同的肺炎克雷伯菌O1:K1血清型菌株中获得的。通过使用肺炎克雷伯菌噬菌体FC3-1、FC3-2和噬菌体phi 1，它们的细菌表面受体分别是脂多糖的O1抗原和K1荚膜多糖（K1），发现在所检测的每个O1:K1菌株中，K1多糖完全覆盖了O1脂多糖分子。仅在亚最小抑菌浓度的抗生素存在下生长后，才观察到细胞表面O抗原的暴露。讨论了这些发现对预防克雷伯菌感染疫苗设计的意义。
