Williams P, Lambert P A, Brown M R
Department of Pharmaceutical Sciences, Aston University, Birmingham.
J Med Microbiol. 1988 May;26(1):29-35. doi: 10.1099/00222615-26-1-29.
The ability of antibodies to cell-surface components of Klebsiella to increase surface hydrophobicity and to gain access to antigens potentially masked by the capsule was investigated. Treatment of capsulate or non-capsulate strains with the respective autologous antiserum resulted in a marked increase in surface hydrophobicity. Antisera raised against a rough non-capsulate (K-O-) strain had little effect on the surface hydrophobicity of either of the capsulate strains K1+O1+ and K2+O1+, or of the non-capsulate K-O1+ strain. Whereas anti-K-O1+ sera or anti-K2+ sera increased the surface hydrophobicity of the K2+O1+ strain, only antisera containing anti-K1+ antibodies increased the hydrophobicity of the K1+O1+ strain. Immunoadsorption of anti-K-O1+ serum by whole capsulate cells revealed that neither the K1 nor the K2 capsular polysaccharide acted as a barrier to anti-O antibodies but that the K1 capsular polysaccharide masked the presence of the immunoglobulin at the cell surface. The Klebsiella capsular polysaccharide does not appear to present a permeability barrier to immunoglobulins although failure to detect outer-membrane proteins in the immune complexes of either of the capsulate strains or of the K-O1+ strain suggests that the O antigen may prevent access of antibodies to these antigens.
研究了抗体与肺炎克雷伯菌细胞表面成分结合后增加表面疏水性以及接触可能被荚膜掩盖的抗原的能力。用各自的自体抗血清处理有荚膜或无荚膜菌株，会导致表面疏水性显著增加。针对粗糙无荚膜（K-O-）菌株产生的抗血清对有荚膜菌株K1+O1+和K2+O1+或无荚膜K-O1+菌株的表面疏水性几乎没有影响。而抗K-O1+血清或抗K2+血清可增加K2+O1+菌株的表面疏水性，只有含有抗K1+抗体的抗血清可增加K1+O1+菌株的疏水性。用完整的有荚膜细胞对抗K-O1+血清进行免疫吸附显示，K1和K2荚膜多糖都不是抗O抗体的屏障，但K1荚膜多糖掩盖了细胞表面免疫球蛋白的存在。肺炎克雷伯菌荚膜多糖似乎不会对免疫球蛋白形成渗透屏障，尽管在有荚膜菌株或K-O1+菌株的免疫复合物中未能检测到外膜蛋白，这表明O抗原可能会阻止抗体接触这些抗原。
