Tomás J M, Camprubi S, Merino S, Davey M R, Williams P
Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Spain.
Infect Immun. 1991 Jun;59(6):2006-11. doi: 10.1128/iai.59.6.2006-2011.1991.
Surface exposure of the O1 serotype lipopolysaccharide in encapsulated Klebsiella pneumoniae strains belonging to different serotypes was examined by using the O1 antigen-specific bacteriophages FC3-1 and FC3-2 in conjunction with immunogold electron microscopy and enzyme immunoassays with specific antisera. Despite the presence of the capsular polysaccharide, the O1 antigen was exposed at the cell surface in strains producing K2, K7, K8, K12, K19, K21, K22, K34, K35, K42, K45, K55, K57, K62, K66, K69, and K70 capsular polysaccharides. However, in strains producing K1, K10, and K16 capsular polysaccharides, the O1 antigen was masked by the K antigen. These results suggest that, since the O1 antigen is surface exposed in many different strains of K. pneumoniae with different capsular serotypes and is also able to immunoprotect, its potential as a useful vaccine component should not be overlooked.
通过使用O1抗原特异性噬菌体FC3-1和FC3-2，结合免疫金电子显微镜和特异性抗血清的酶免疫测定，检测了不同血清型的肺炎克雷伯菌荚膜菌株中O1血清型脂多糖的表面暴露情况。尽管存在荚膜多糖，但在产生K2、K7、K8、K12、K19、K21、K22、K34、K35、K42、K45、K55、K57、K62、K66、K69和K70荚膜多糖的菌株中，O1抗原暴露于细胞表面。然而，在产生K1、K10和K16荚膜多糖的菌株中，O1抗原被K抗原掩盖。这些结果表明，由于O1抗原在许多具有不同荚膜血清型的肺炎克雷伯菌不同菌株中都暴露于表面，并且还具有免疫保护作用，因此其作为有用疫苗成分的潜力不应被忽视。
