Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology,Northwest A&F University, Yangling, Shaanxi 712100, China.
Department of Animal Genetics and Breeding, College of Animal Science and Technology, Key Laboratoryof Animal Genetics and Breeding of Ministry of Agriculture, National Engineering Laboratory for AnimalBreeding, China Agricultural University, Beijing 100193, China.
Genes (Basel). 2019 Jan 30;10(2):104. doi: 10.3390/genes10020104.
Heat shock 70 kDa protein 8 (HSPA8) and erb-b2 receptor tyrosine kinase 2 (ERBB2) were the promising candidates for milk protein concentration in dairy cattle revealed through previous RNA sequencing (RNA-Seq) study. The objective of this post-RNA-Seq study was to confirm genetic effects of HSPA8 and ERBB2 on milk protein concentration in a large Chinese Holstein population and to evaluate the genetic effects of both genes on other milk production traits. There were 2 singlenucleotide polymorphisms (SNPs) identified for HSPA8 and 11 SNPs for ERBB2 by sequencing 17 unrelated Chinese Holstein sires. The SNP-rs136632043 in HSPA8 had significant associations with all five milk production traits (p = 0.0086 to p < 0.0001), whereas SNP-rs132976221 was remarkably associated with three yield traits (p < 0.0001). Nine (ss1996900615, rs109017161, rs109122971, ss1996900614, rs110133654, rs109941438, rs110552983, rs133031530, and rs109763505) of 11 SNPs in ERBB2 were significantly associated with milk protein percentage (p = 0.0177 to p < 0.0001). A 12 Kb haplotype block was formed in ERBB2 and haplotype associations revealed similar effects on milk protein traits. Our findings confirmed the significant genetic effects of HSPA8 and ERBB2 on milk protein concentration and other milk production traits and SNP phenotypic variances above 1% may serve as genetic markers in dairy cattle breeding programs.
热休克 70kDa 蛋白 8（HSPA8）和表皮生长因子受体 2（ERBB2）是先前 RNA 测序（RNA-Seq）研究中发现的具有潜力的奶牛乳蛋白浓度候选基因。本 RNA-Seq 后研究的目的是在大型中国荷斯坦牛群体中确认 HSPA8 和 ERBB2 对乳蛋白浓度的遗传效应，并评估这两个基因对其他产奶性状的遗传效应。通过对 17 头无关中国荷斯坦公牛进行测序，共鉴定出 HSPA8 的 2 个单核苷酸多态性（SNP）和 ERBB2 的 11 个 SNP。HSPA8 中的 SNP-rs136632043 与所有 5 个产奶性状均显著相关（p = 0.0086 至 p < 0.0001），而 SNP-rs132976221 与 3 个产奶性状显著相关（p < 0.0001）。ERBB2 中的 11 个 SNP 中有 9 个（ss1996900615、rs109017161、rs109122971、ss1996900614、rs110133654、rs109941438、rs110552983、rs133031530 和 rs109763505）与乳蛋白百分比显著相关（p = 0.0177 至 p < 0.0001）。在 ERBB2 中形成了一个 12 Kb 的单倍型块，单倍型关联揭示了对乳蛋白性状的相似影响。我们的研究结果证实了 HSPA8 和 ERBB2 对乳蛋白浓度和其他产奶性状的显著遗传效应，且表型方差超过 1%的 SNP 可能作为奶牛育种计划中的遗传标记。
