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长期 pH 值对反硝化的影响:高 pH 值有利于实现部分反硝化。

Long-term effect of pH on denitrification: High pH benefits achieving partial-denitrification.

机构信息

National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China; Zhejiang Province Institute of Architectural Design and Research, Hangzhou 310006, China.

College of Environment and Ecology, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China; National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China.

出版信息

Bioresour Technol. 2019 Apr;278:444-449. doi: 10.1016/j.biortech.2019.01.105. Epub 2019 Jan 23.

Abstract

Partial-denitrification (nitrate to nitrite) can supply nitrite for anammox which can reduce organic matter consumption in wastewater treatment plants (WWTPs). In order to achieve stable partial-denitrification, the effect of pH on denitrification were investigated for 420 days in three reactors with influent pH of 5.0, 7.0 and 9.0. The results indicate that the nitrite accumulation rate (NAR) increased with pH, with average effluent NARs being 21%, 38% and 57% in the above reactors, respectively. The sludge cultivated at a high pH of 9.0 was resistant to pH shock, with a high NAR being maintained at 83% when it was exposed to a low pH of 5.0. Metagenomic analysis showed that the higher NAR at pH 9.0 was correlated with an enrichment of Thauera, which harbored more nitrate reductase (8098 hits) than nitrite reductase (2950 hits). Based on these findings, a novel process was proposed for achieving partial-denitrification/anammox in mainstream WWTPs.

摘要

部分反硝化(硝酸盐至亚硝酸盐)可以为厌氧氨氧化提供亚硝酸盐,从而减少污水处理厂(WWTP)中的有机物消耗。为了实现稳定的部分反硝化,在三个进水 pH 值分别为 5.0、7.0 和 9.0 的反应器中进行了 420 天的研究,考察了 pH 值对反硝化的影响。结果表明,亚硝酸盐积累率(NAR)随 pH 值的升高而增加,上述三个反应器的平均出水 NAR 分别为 21%、38%和 57%。在高 pH 值 9.0 下培养的污泥对 pH 值冲击具有抗性,当暴露于低 pH 值 5.0 时,仍能保持 83%的高 NAR。宏基因组分析表明,在 pH 值 9.0 时 NAR 较高与 Thauera 的富集有关,该菌含有更多的硝酸盐还原酶(8098 个命中)而不是亚硝酸盐还原酶(2950 个命中)。基于这些发现,提出了一种在主流 WWTP 中实现部分反硝化/厌氧氨氧化的新工艺。

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