He Chuan, Wei Li'e, Lai Faying, Zhou Chunhuo, Ni Guorong, Hu Jianmin, Yin Xin
Nanchang Key Laboratory of Nutrition Management of Crops, Prevention and Controlling of Agricultural Non-point Source Pollution, College of Land Resource and Environment, Jiangxi Agricultural University Nanchang 330045 PR China
Jiangxi Provincial Key Laboratory of Water Resources and Environment of Poyang Lake, Jiangxi Institute of Water Sciences Nanchang 330029 PR China.
RSC Adv. 2019 Dec 13;9(70):41351-41360. doi: 10.1039/c9ra05525h. eCollection 2019 Dec 9.
In this study, immobilizing partial denitrification biomass and redox mediators to integrate with the anammox process for nitrogen removal was investigated. Three redox mediators (RMs), namely, 2-methyl-1,4-naphthoquinone (ME), anthraquinone (AQ) and 1-dichloroanthraquinone (1-AQ) were catalyzed to reduce nitrate to only nitrite by denitrification to integrate with the anammox process for nitrogen removal. First, our experimental results showed that there were 35.8, 42.2 and 53.0 mg-N L nitrite accumulation values with the addition of ME, AQ and 1-AQ, respectively, at the dose of 75 µM by the denitrification process at C/N = 2, which were 25.6%, 48.2% and 86.1% higher than that of the control without the addition of any RMs. Nitrate reductase activities were higher than that of nitrite reductase affected by RMs, which was the main reason for nitrite accumulation and further maintenance of the anammox process. Second, owing to the stable nitrite production by the partial denitrifying biomass with the addition of 1-AQ, the nitrogen removal rate of the reactor that integrated the partial denitrification and anammox process reached 1788.36 g-N m d only using ammonia and nitrate as the influent nitrogen resource in the long-term operation. Third, the 16S rDNA sequencing results demonstrated that and were the primary groups of the denitrifying biomass, which were considered the dominant partial denitrification species.
在本研究中，对固定化部分反硝化生物质和氧化还原介质以与厌氧氨氧化过程相结合进行脱氮进行了研究。三种氧化还原介质（RMs），即2-甲基-1,4-萘醌（ME）、蒽醌（AQ）和1,2-二氯蒽醌（1-AQ），通过反硝化作用被催化将硝酸盐仅还原为亚硝酸盐，以与厌氧氨氧化过程相结合进行脱氮。首先，我们的实验结果表明，在C/N = 2时，通过反硝化过程，在添加75 µM的ME、AQ和1-AQ时，亚硝酸盐积累值分别为35.8、42.2和53.0 mg-N/L，比不添加任何RMs的对照组分别高出25.6%、48.2%和86.1%。受RMs影响，硝酸盐还原酶活性高于亚硝酸盐还原酶活性，这是亚硝酸盐积累以及厌氧氨氧化过程得以进一步维持的主要原因。其次，由于添加1-AQ后部分反硝化生物质能稳定产生亚硝酸盐，在长期运行中，将部分反硝化和厌氧氨氧化过程相结合的反应器仅以氨和硝酸盐作为进水氮源时，脱氮率达到1788.36 g-N/(m³·d)。第三，16S rDNA测序结果表明，[具体微生物名称未给出]和[具体微生物名称未给出]是反硝化生物质的主要菌群，它们被认为是主要的部分反硝化物种。
