Bhowmik Deepshikha, Chetri Shiela, Paul Deepjyoti, Chanda Debadatta Dhar, Bhattacharjee Amitabha
Junior Research Fellow (Microbiology), Assam University, Silchar, Assam, India.
Senior Research Fellow (Microbiology), Assam University, Silchar, Assam, India.
Med J Armed Forces India. 2019 Jan;75(1):86-89. doi: 10.1016/j.mjafi.2018.08.004. Epub 2018 Oct 25.
In , methicillin resistance is exhibited by modifications in penicillin-binding protein that minimises the binding affinity to beta-lactam antibiotics. The present study investigated the occurrence of methicillin-resistant . (MRSA) in community-acquired infections, that is, community-acquired MRSA (CA-MRSA) and in-hospital-acquired infections, that is, hospital-acquired MRSA (HA-MRSA) from Northeast India.
A total of 197 consecutive non-duplicate isolates were collected from Silchar Medical College and Hospital and other private diagnostic laboratories. The isolates were confirmed to be at our centre. All isolates were subjected to antibiotic susceptibility testing and were screened for methicillin resistance using cefoxitin disc test. All MRSA were subjected to Polymerase Chain Reaction (PCR) assay for detection of and genes. DNA fingerprinting was performed for determining clonal diversity.
Seventy-one isolates of 127 confirmed . were found to be methicillin resistant by screening test. gene was detected in 43 isolates, and none of the isolates were positive for gene. Linezolid and teicoplanin showed better activity with susceptibility pattern being 83.6% and 72.44%, respectively, whereas 66.14% were sensitive to vancomycin. Other antibiotic showed low level of activity. Pulsed Field Gel Electrophoresis (PFGE) showed 14 different banding patterns that suggest isolates were of different clonal types.
was responsible for methicillin resistance in majority of strains. Polyclonal spread of MRSA infection in the study area indicates its diverse origin and possible lateral transfer. Thus, this study is of clinical interest in terms of selection of proper antimicrobial chemotherapy and infection control management.
在[具体细菌名称未给出]中，对甲氧西林耐药是通过青霉素结合蛋白的修饰来实现的，这种修饰会使与β-内酰胺类抗生素的结合亲和力降至最低。本研究调查了印度东北部社区获得性感染中耐甲氧西林[具体细菌名称未给出]（MRSA）的发生情况，即社区获得性MRSA（CA-MRSA）以及医院获得性感染中耐甲氧西林[具体细菌名称未给出]（HA-MRSA）的发生情况。
从锡尔恰尔医学院和医院以及其他私人诊断实验室共收集了197株连续的非重复分离株。这些分离株在我们中心被确认为[具体细菌名称未给出]。所有分离株均进行了抗生素敏感性测试，并使用头孢西丁纸片扩散法筛选甲氧西林耐药性。所有MRSA均进行聚合酶链反应（PCR）检测以检测[具体基因名称未给出]和[具体基因名称未给出]基因。进行DNA指纹图谱分析以确定克隆多样性。
在127株确诊的[具体细菌名称未给出]中，有71株分离株经筛选试验发现对甲氧西林耐药。在43株分离株中检测到[具体基因名称未给出]基因，且没有分离株[具体基因名称未给出]基因呈阳性。利奈唑胺和替考拉宁显示出较好的活性，敏感性分别为83.6%和72.44%，而66.14%的菌株对万古霉素敏感。其他抗生素显示出较低的活性水平。脉冲场凝胶电泳（PFGE）显示出14种不同的条带模式，表明分离株属于不同的克隆类型。
[具体细菌名称未给出]是大多数菌株对甲氧西林耐药的原因。研究区域内MRSA感染的多克隆传播表明其来源多样且可能存在横向转移。因此，本研究在选择合适的抗菌化疗和感染控制管理方面具有临床意义。
