Department of Chemistry, University of Girona, C/M. Aurèlia Campmany 69, 17003, Girona, Spain.
Department of Analytical Chemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, A. Kovacica 1, 10000, Zagreb, Croatia.
Anal Bioanal Chem. 2019 Mar;411(8):1659-1670. doi: 10.1007/s00216-019-01618-3. Epub 2019 Feb 1.
Multielemental analysis of whole blood can provide significant information for the evaluation of nutritional status and diagnosis of certain diseases as well as for the assessment of exposure to potentially toxic metals. However, the quantification of multiple elements in whole blood is not easy partly because of the wide variation in element concentrations (from ng L to g L) and the complex matrix. The aim of this work was to develop a fast, sustainable, and reliable analytical method, in combination with low-power TXRF, for multielemental analysis of blood samples. Firstly, a set of experiments were carried out to select the best diluent type and dilution factor using the control material SeronormTM Trace Elements Whole Blood L-1. A critical evaluation of the parameters affecting the sample deposition on the reflector was also carried out including a study of the shape and element distribution of the deposited residue on the reflector by micro X-ray fluorescence spectrometry. Using the best analytical conditions, limits of detection estimated were in the low milligrams per kilogram range and similar to those obtained using more complex sample treatments such as digestion. Accuracy and precision of the results were in most cases acceptable (recoveries 89-102%, RSD 6-8%, n = 5). Only underestimated values were obtained for light elements such as potassium. To prove the applicability of the method, several blood samples from control and thyroid disease patients were analyzed. Despite the fact that more samples need to be analyzed, it seems that Zn and Br contents in some of the patients are significantly higher compared to control samples. Graphical abstract.
全血的多元素分析可以提供重要的信息，用于评估营养状况和诊断某些疾病，以及评估暴露于潜在有毒金属的情况。然而，全血中多种元素的定量并不容易，部分原因是元素浓度（从 ng/L 到 g/L）变化范围广，基质复杂。本工作旨在结合低功率 TXRF 开发一种快速、可持续和可靠的分析方法，用于全血样品的多元素分析。首先，进行了一系列实验，使用对照材料 SeronormTM Trace Elements Whole Blood L-1 选择最佳稀释剂类型和稀释因子。还对影响反射器上样品沉积的参数进行了严格评估，包括通过微 X 射线荧光光谱法研究沉积在反射器上的残留物的形状和元素分布。使用最佳分析条件，估计的检出限在毫克/千克的低范围内，与使用更复杂的样品处理（如消化）获得的检出限相似。在大多数情况下，结果的准确性和精密度是可以接受的（回收率 89-102%，RSD 6-8%，n=5）。只有钾等轻元素的测定值被低估。为了证明该方法的适用性，分析了来自对照和甲状腺疾病患者的几个血样。尽管需要分析更多的样本，但似乎一些患者的 Zn 和 Br 含量明显高于对照样本。图表摘要。
