Kusuma School of Biological Sciences, Indian Institute of Technology, Hauz Khas, New Delhi, India.
Dept. of Pediatrics, All India Institute of Medical Sciences, Ansari nagar, New Delhi, India.
PLoS One. 2019 Feb 1;14(2):e0211036. doi: 10.1371/journal.pone.0211036. eCollection 2019.
Chikungunya virus (CHIKV) infection is a long known mosquito-borne disease that is associated with severe morbidity, characterized by fever, headache, rashes, joint pain, and myalgia. It is believed that virus load has relation with severity of clinical features.
We performed this study to assess the relationship between virus load and clinical features in children during the acute phase of CHIKV infection, in order to draw insights for better-informed treatment.
Between June 1, 2009, and May 31, 2010, 338 patients with fever and susceptive to CHIKV during first 4 days of illness were prospectively enrolled from Karnataka Institute of Medical Sciences, Hubli in our hospital based cross sectional observational study. Sybr green quantitative reverse transcription polymerase chain reaction was performed to estimate the virus load.
Quantitative RT-PCR was positive for CHIKV in 54 patients. The median copy number of CHIKV was 1.3x 108 copies/ml (1.7x105-9.9x109 copies/ml). Among the observed clinical features, a statistically significant difference in log mean virus load was found between patients with and without myalgia (log mean 7.50 vs 8.34, P = 0.01).
Patients with myalgia had lower virus load and those without myalgia had a higher virus load.
基孔肯雅热病毒（CHIKV）感染是一种长期以来已知的蚊媒疾病，与严重的发病率有关，其特征是发热、头痛、皮疹、关节痛和肌痛。据信病毒载量与临床特征的严重程度有关。
我们进行这项研究是为了评估儿童在基孔肯雅热病毒感染的急性期病毒载量与临床特征之间的关系，以便为更明智的治疗提供见解。
2009 年 6 月 1 日至 2010 年 5 月 31 日，我们在医院进行了一项基于横断面观察性研究，前瞻性招募了来自卡纳塔克邦医学科学研究所，胡布利的 338 名发热且在发病的前 4 天内易感染基孔肯雅热病毒的患者。采用 Sybr 绿色定量逆转录聚合酶链反应估计病毒载量。
54 例患者的定量 RT-PCR 呈基孔肯雅热病毒阳性。CHIKV 的中位拷贝数为 1.3x108 拷贝/ml（1.7x105-9.9x109 拷贝/ml）。在所观察到的临床特征中，患有和不患有肌痛的患者的 CHIKV 病毒载量的对数平均值存在统计学显著差异（对数平均值分别为 7.50 和 8.34，P=0.01）。
患有肌痛的患者病毒载量较低，而不患有肌痛的患者病毒载量较高。
