High-throughput sequencing reveals rhizosphere fungal community composition and diversity at different growth stages of in the lower reaches of the Tarim River.

BACKGROUND

is one of the most ancient and primitive tree species of and plays an important role in maintaining the ecological balance in desert areas. To decipher the diversity, community structure, and relationship between rhizosphere fungi and environmental factors at different growth stages of demands an in-depth investigation.

METHODS

In this study, at different growth stages (young, medium, overripe, and decline periods) was selected as the research object, based on the determination of the physicochemical properties of its rhizosphere soil, the fungal community structure and diversity of and their correlation with soil physicochemical properties were comprehensively analyzed through high-throughput sequencing technology (internal transcribed spacer (ITS)) and bioinformatics analysis methods.

RESULTS

According to the analysis of OTU annotation results, the rhizosphere soil fungal communities identified in were categorized into10 phyla, 36 classes, 77 orders, 165 families, 275 genera and 353 species. The alpha diversity analysis showed that there was no obvious change between the different growth stages, while beta diversity analysis showed that there were significantly differences in the composition of rhizosphere soil fungal communities between mature and overripe trees ( = 0.31, = 0.001), mature and deadwood ( = 0.28, = 0.001). Ascomycota and Basidiomycota were dominant phyla in the rhizosphere fungal community and the dominant genera were and The relative abundance of the top ten fungi at each classification level differed greatly in different stages. Canonical correspondence analysis (CCA) and Spearman's correlation analysis showed that conductivity (EC) was the main soil factor affecting the composition of rhizosphere soil fungal community ( < 0.01), followed by total dissolvable salts (TDS) and available potassium (AK) ( < 0.05).

CONCLUSIONS

Our data revealed that the rhizosphere fungal communities at the different growth stages of have differences, conductivity (EC) was the key factor driving rhizosphere fungi diversity and community structure, followed by total dissolvable salts (TDS) and available potassium (AK).