新疆猪毛菜水溶性多糖的提取、分离纯化及结构特征
A water-soluble polysaccharide from the roots of Eremurus spectabilis M. B. subsp. spectabilis: Extraction, purification and structural features.
机构信息
Shahrood University of Technology, Faculty of Agriculture, Department of Food Science and Technology, Shahrood, Iran.
Shahrood University of Technology, Faculty of Agriculture, Department of Food Science and Technology, Shahrood, Iran.
出版信息
Int J Biol Macromol. 2019 May 1;128:648-654. doi: 10.1016/j.ijbiomac.2019.01.178. Epub 2019 Jan 30.
A heteropolysaccharide ESP, was separated from a water extract of dried roots of Eremurus spectabilis by column chromatographies on DEAE-cellulose A52 and Sephadex G-100. Specific optical rotation, molecular mass and total carbohydrate content of ESP were -36.3°, 43.8 kDa and 97.2%, respectively. Monosaccharide composition analysis showed that ESP consisted of glucose and mannose at a molar ratio of 1.0:2.9. Structural characterization of ESP was done by various chemical and instrumental techniques (methylation, partial acid hydrolysis, Smith degradation, periodate oxidation, GLC-MS and 1D/2D NMR). The repeating unit of ESP was determined as following.
从新疆鹅绒藤(Eremurus spectabilis)干根的水提物中分离得到一种杂多糖 ESP，通过 DEAE-纤维素 A52 和 Sephadex G-100 柱层析进行分离。ESP 的比旋光度、分子量和总糖含量分别为-36.3°、43.8 kDa 和 97.2%。单糖组成分析表明，ESP 由葡萄糖和甘露糖组成，摩尔比为 1.0:2.9。通过各种化学和仪器技术（甲基化、部分酸水解、Smith 降解、高碘酸氧化、GLC-MS 和 1D/2D NMR）对 ESP 的结构进行了表征。ESP 的重复单元如下。
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