Shahrood University of Technology, School of Agricultural Engineering, Department of Food Science and Technology, Shahrood, Iran.
Department of Polymer Engineering, Science and Research Branch, Islamic Azad University, 14515-775, Tehran, Iran.
Carbohydr Polym. 2017 Dec 15;178:386-393. doi: 10.1016/j.carbpol.2017.09.058. Epub 2017 Sep 18.
A new water-soluble polysaccharide (ESPS-1), with the average molecular weight of 52.1kDa and a specific optical rotation of +169.7° (c 1.0, HO), was successfully isolated by warm-water extraction and then purified using DEAE-Cellulose and Sephadex G-100 columns from the roots of Eremurus stenophyllus. The results from chemical and instrumental tests including FT-IR, methylation and GC-MS analysis, periodate oxidation and Smith degradation, partial acid hydrolysis, and NMR spectroscopy (H, C, H-H COSY, HMQC and HMBC) demonstrated that ESPS-1 was a 2-O-acetylgalactan, composed of galactose, arabinose and mannose in a molar ratio of about 10:3:1, respectively. It has a backbone of (1→6)-α-d-galactopyranosyl residues, with branches at O-3 consisting of α-d-Manp-(1→3)-α-l-Araf-(1→3)-α-l-Araf-(1→3)-α-l-Araf-(1→. The acetyl groups are substituted at O-2 of (1→6)-linked Galp and the degree of acetylation was about 14%.
从长叶松叶菊(Eremurus stenophyllus)的根部，经温水提取、DEAE-纤维素柱层析和 Sephadex G-100 凝胶柱层析分离纯化，得到一种新的水溶性多糖(ESPS-1)。ESPS-1 的平均分子量为 52.1 kDa，比旋光度为+169.7°(c 1.0，HO)。其化学和仪器测试结果，包括傅里叶变换红外光谱(FT-IR)、甲基化和气相色谱-质谱联用(GC-MS)分析、高碘酸氧化和 Smith 降解、部分酸水解以及核磁共振波谱(H、C、H-H COSY、HMQC 和 HMBC)表明，ESPS-1 是一种 2-O-乙酰基半乳糖，由半乳糖、阿拉伯糖和甘露糖以摩尔比约 10:3:1 组成。它的主链由(1→6)-α-d-半乳糖吡喃糖基残基组成，在 O-3 处的支链由α-d-Manp-(1→3)-α-l-Araf-(1→3)-α-l-Araf-(1→3)-α-l-Araf-(1→组成。乙酰基取代(1→6)-连接的 Galp 的 O-2，乙酰化程度约为 14%。
