Key Laboratory of Science and Technology of Eco-Textiles, Ministry of Education, Donghua University, Shanghai, 201620, China.
Key Laboratory of Science & Technology of Eco-Textiles, Ministry of Education, Jiangnan University, 1800 Lihu Road, Wuxi, Jiangsu, 214122, China.
Colloids Surf B Biointerfaces. 2019 May 1;177:33-40. doi: 10.1016/j.colsurfb.2019.01.043. Epub 2019 Jan 24.
Keratin films with wet stability and strength suitable for biomedical applications were developed via reinforcement with submicron cysteine particles for improved interfaces. Keratin products regenerated from wool or human hair were widely investigated as wound dressing and tissue engineering scaffolds for their satisfactory biomedical properties. However, regenerated keratin scaffolds usually did not have good mechanical properties, and also could not stand humid or wet biological environment due to poor moisture stability. Reinforcements for keratin materials were usually polysaccharides or synthetic polymers, and thus usually had non-ideal interfacial properties due to limited compatibility. In this research, submicron cystine particles were employed to reinforce keratin films for their high compatibility with keratin and bio-safety. Transition of primary and secondary structures of keratin due to matrix-reinforcement interaction was analyzed. The keratin films showed unprecedented pliancy, good tensile properties under humid conditions and biocompatibility, and thus had good potential for biomedical engineering applications.
通过添加亚微米半胱氨酸颗粒来增强界面，开发出具有湿稳定性和强度的适合生物医学应用的角蛋白薄膜。角蛋白产品由羊毛或人发再生，因其满意的生物医学特性而被广泛用作伤口敷料和组织工程支架。然而，由于机械性能差，再生角蛋白支架通常不能耐受潮湿或湿润的生物环境。角蛋白材料的增强剂通常是多糖或合成聚合物，因此由于相容性有限，通常界面性能不理想。在这项研究中，使用亚微米胱氨酸颗粒来增强角蛋白薄膜，因为它们与角蛋白具有很高的相容性和生物安全性。由于基质增强相互作用，角蛋白的一级和二级结构发生了转变。角蛋白薄膜具有前所未有的柔韧性、在潮湿条件下的良好拉伸性能和生物相容性，因此具有很好的生物医学工程应用潜力。
