HPLC-ESI-MS Identification and NMR Characterization of Glucosyloxybenzyl 2-Benzylmalate Deriva-Tives from and Their Anti-Liver Fibrotic Effects In Vitro.

Four new glucosyloxybenzyl 2-benzylmalate derivatives, named Arundinoside H (), I (), J (), K () as well as four known compounds Arundinoside D (), G (), F (), E () were isolated and characterized by a combination of chemical and spectroscopic methods, including HR-ESI-MS, 1D and 2D NMR experiments. Besides, 24 unreported compounds were inferred from ESI-MS data. The anti-liver fibrotic activities of the isolates were determined as proliferation inhibition of lipopolysaccharide (LPS)-induced activation of rat hepatic stellate cells (HSC-T6). The result suggested Arundinosides D, H, F, I and K showed moderate inhibitory effects in vitro.