National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
Bioresour Technol. 2019 Apr;278:468-472. doi: 10.1016/j.biortech.2019.01.056. Epub 2019 Jan 15.
This study investigated the nitritation of domestic wastewater through the short-term addition of hydroxylamine (NHOH). Sequencing batch reactor (SBR) was used and the NHOH solution with an initial concentration of 5.0 mg/L was injected at each cycle. With NHOH addition, the nitritation was quickly established in 5 d with nitrite accumulation ratio above 95%. Further, stable nitritation could be maintained without NHOH addition in the following 53 days, even under unfavorable conditions (DO = 3 mg/L). According to qPCR results, NHOH significantly reduced and continuously suppressed nitrite oxidizing bacteria (NOB), while the abundance of ammonia oxidizing bacteria was stable, induced the start-up and maintenance of nitritation. Among NOB, NHOH significantly suppressed Nitrospira, while did not affect Nitrobactor. Nitrobactor gradually increased during the operation, which could induce the final deterioration of nitritation. Overall, this research provided the fundamental knowledge required to optimize the NHOH addition strategy for operating a stable nitritation in domestic wastewater.
本研究通过短期添加羟胺（NHOH）来研究生活污水的亚硝化作用。采用序批式反应器（SBR），每个周期注入初始浓度为 5.0mg/L 的 NHOH 溶液。通过添加 NHOH，在 5 天内迅速建立了亚硝化作用，亚硝酸盐积累率超过 95%。进一步，在随后的 53 天内，即使在不利条件下（DO=3mg/L），也无需添加 NHOH 就可以维持稳定的亚硝化作用。根据 qPCR 结果，NHOH 显著减少并持续抑制亚硝酸氧化菌（NOB），而氨氧化菌的丰度保持稳定，诱导了亚硝化作用的启动和维持。在 NOB 中，NHOH 显著抑制了 Nitrospira，而对 Nitrobacter 没有影响。在运行过程中，Nitrobacter 逐渐增加，这可能导致亚硝化作用的最终恶化。总的来说，这项研究为优化生活污水中稳定亚硝化作用的 NHOH 添加策略提供了必要的基础知识。
