Qiao Xin, Wang Bo, Guo Yuan-Yuan, Peng Yong-Zhen
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Key Laboratory of Beijing for Water Quality Science and Water Environment Recovery Engineering, Beijing University of Technology, Beijing 100124, China.
Xinkai Water Environmental Investment Co., Ltd., Beijing 101101, China.
Huan Jing Ke Xue. 2020 Aug 8;41(8):3765-3772. doi: 10.13227/j.hjkx.201911190.
The effective inhibition of nitrite oxidizing bacteria (NOB) is the key to realizing satisfactory nitrite accumulation and achieving effective nitritation. In order to explore the selective effect of hydroxylamine (NH OH) on ammonia oxidizing bacteria (AOB) and NOB, a sequencing batch reactor (SBR) with the operation mode of anaerobic/aerobic/anoxia (A/O/A) was used to observe the start-up of nitritation at different concentrations and frequencies of NH OH. The results showed that when 5 mg·L of NH OH was added once every 2 cycles, the nitrite accumulation rate (NAR) increased from 0.1% to 57.4% in 6 days, and was maintained at (62.0±4.6)% until the end of the trials. In the typical cycle on day 6, the NN-N dropped from 26.05 mg·L to 8.06 mg·L, thus producing 9.02 mg·L of NO-N and 6.70 mg·L of NO-N. Meanwhile, the ratio of the maximum activity of AOB (rAOB) to NOB (rNOB) increased from 1.05 on day 1 to 4.22 on day 9. Moreover, qPCR results indicated that the abundance of AOB and NOB decreased to 30.2% and 19.1%, respectively, on day 9 in comparison to the original sample. The results indicate that the selective effect of AOB and NOB based on NH OH is expected to provide a feasible application for the rapid start-up nitritation of municipal wastewater.
有效抑制亚硝酸盐氧化细菌（NOB）是实现令人满意的亚硝酸盐积累和有效亚硝化的关键。为了探究羟胺（NH₂OH）对氨氧化细菌（AOB）和NOB的选择性作用，采用厌氧/好氧/缺氧（A/O/A）运行模式的序批式反应器（SBR）来观察不同浓度和投加频率的NH₂OH条件下亚硝化的启动情况。结果表明，当每2个周期投加一次5 mg·L⁻¹的NH₂OH时，亚硝酸盐积累率（NAR）在6天内从0.1%增至57.4%，并在试验结束前维持在（62.0±4.6）%。在第6天的典型周期中，总氮（TN-N）从26.05 mg·L⁻¹降至8.06 mg·L⁻¹，从而产生了9.02 mg·L⁻¹的亚硝酸盐氮（NO₂-N）和6.70 mg·L⁻¹的硝酸盐氮（NO₃-N）。同时，AOB的最大活性与NOB的最大活性之比（rAOB/rNOB）从第1天的1.05增至第9天的4.22。此外，定量聚合酶链反应（qPCR）结果表明，与原始样品相比，第9天AOB和NOB的丰度分别降至30.2%和19.1%。结果表明，基于NH₂OH对AOB和NOB的选择性作用有望为城市污水亚硝化的快速启动提供可行的应用方案。
