Chongqing Engineering Research Center of Specialty Crop Resources and The College of Life Science, Chongqing Normal University, Chongqing 401331, China; The School of Life Science, Chongqing University, Chongqing 401331, China.
Chongqing Engineering Research Center of Specialty Crop Resources and The College of Life Science, Chongqing Normal University, Chongqing 401331, China.
Fungal Genet Biol. 2019 May;126:25-36. doi: 10.1016/j.fgb.2019.01.009. Epub 2019 Jan 30.
Verticillium dahliae is a soil-borne, hemibiotrophic phytopathogenic fungus that causes Verticillium wilt in a broad range of economic crops. The microsclerotia (MS), which act as the main host inoculum, can survive long-term in soil resulting in uncontrollable disease. In order to clarify the mechanism of MS formation, we sequenced the whole genome-wide expression profile of V. dahliae strain V991. Compared with M1 (no MS formation), during the process of MS formation and maturation, 1354, 1571, and 1521 unique tags were significantly regulated in M2, M3, and M4 library, respectively. During MS formation, melanin synthesis-related genes were preferentially upregulated. The process is more likely to regulated by transcription factors (TFs) including CH, ZnCys, bZIP, and fungal-specific TF domain-containing proteins; additionally, G-protein coupled receptors, Ca, small GTPases, and cAMP were involved in signalling transduction. Protein kinase-encoding (VDAG_06474) and synthase-encoding (VDAG_05314) genes were demonstrated to negatively and positively influence MS production, respectively. The gene expression dynamics revealed during MS formation provide comprehensive theoretical knowledge to further understanding of the metabolism and regulation of MS development in V. dahliae, potentially providing targets to control Verticillium wilt through interfering MS formation.
轮枝菌是一种土壤传播、半活体营养的植物病原真菌，可引起广泛的经济作物黄萎病。微菌核（MS）作为主要的宿主接种体，可以在土壤中长期存活，导致疾病无法控制。为了阐明 MS 形成的机制，我们对 V. dahliae 菌株 V991 进行了全基因组表达谱测序。与 M1（无 MS 形成）相比，在 MS 形成和成熟过程中，M2、M3 和 M4 文库中分别有 1354、1571 和 1521 个独特标签被显著调控。在 MS 形成过程中，黑色素合成相关基因被优先上调。这一过程更可能受到包括 CH、ZnCys、bZIP 和真菌特异性 TF 结构域蛋白在内的转录因子（TFs）的调控；此外，G 蛋白偶联受体、Ca、小 GTPases 和 cAMP 参与信号转导。蛋白激酶编码基因（VDAG_06474）和合成酶编码基因（VDAG_05314）分别被证明对 MS 产量有负向和正向影响。MS 形成过程中的基因表达动态为进一步理解 V. dahliae 中 MS 发育的代谢和调控提供了全面的理论知识，可能为通过干扰 MS 形成来控制黄萎病提供了目标。
