两株猪腰豆蛋白酶抑制剂对土垄大白蚁及其生存的肠道酶的不同特异性的影响。
Effects of two protease inhibitors from Bauhinia bauhinoides with different specificity towards gut enzymes of Nasutitermes corniger and its survival.
机构信息
Biochemistry Department at the Federal University of São Paulo, 04044-020, São Paulo, SP, Brazil.
Biochemistry Department at the Federal University of Pernambuco, 50670-420, Recife, PE, Brazil.
出版信息
Chemosphere. 2019 May;222:364-370. doi: 10.1016/j.chemosphere.2019.01.108. Epub 2019 Jan 21.
Two recombinant protease inhibitors from Bauhinia bauhinioides, rBbKI (kallikrein inhibitor) and rBbCI (cruzipain inhibitor) were evaluated for insecticidal activity against workers and soldiers of Nasutitermes corniger (order: Isoptera; family: Termitidae) through the inhibitors' effect on the insect's gut enzymes. The inhibitor rBbKI was more effective than rBbCI in inhibiting the termite's gut enzymes. The kallikrein inhibitor showed termiticidal activity in workers with an LC of 0.9 mg mL after 4 days. Conversely, rBbKI did not affect the survival of soldiers and rBbCI did not show termiticidal activity against N. corniger. The two inhibitors showed different specificity towards the termite's gut enzymes, representing interesting tools to characterize N. corniger enzymes. The different effects of rBbKI and rBbCI on the termite's enzymes and survival may be linked to slight structural differences between these inhibitors.
从紫荆中分离得到的两种重组蛋白酶抑制剂 rBbKI（激肽释放酶抑制剂）和 rBbCI（克氏锥虫抑制剂），通过抑制昆虫肠道酶的作用，评估其对玉米象鼻虫工蚁和兵蚁的杀虫活性。抑制剂 rBbKI 比 rBbCI 更能有效抑制白蚁肠道酶。在第 4 天，这种激肽释放酶抑制剂对工蚁的 LC 为 0.9mg/mL，表现出杀白蚁活性。相反，rBbKI 不影响兵蚁的存活，rBbCI 对玉米象鼻虫也没有杀虫活性。这两种抑制剂对白蚁肠道酶表现出不同的特异性，为研究玉米象鼻虫的酶提供了有价值的工具。rBbKI 和 rBbCI 对白蚁酶和存活的不同影响可能与其结构上的细微差异有关。
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