基于切口酶驱动的 DNA 行走机器的简单灵敏的电化学发光适体传感器用于检测赭曲霉毒素 A。
A simple and sensitive electrochemiluminescence aptasensor for determination of ochratoxin A based on a nicking endonuclease-powered DNA walking machine.
机构信息
College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China; Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
Jiangsu Province Hi-Tech Key Laboratory for Bio-medical Research, Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
出版信息
Food Chem. 2019 Jun 1;282:141-146. doi: 10.1016/j.foodchem.2019.01.011. Epub 2019 Jan 11.
Ochratoxin A (OTA) poses a serious threat to the health of human beings and animals. In this paper, a simple and sensitive electrochemiluminescence (ECL) aptasensor was constructed to detect OTA based on electrochemiluminescence resonance energy transfer (ECL-RET) and a nicking endonuclease-powered DNA walking machine. Originally, the signal of cadmium sulfide semiconductor quantum dots (CdS QDs) was quenched efficiently by Cy5. After the addition of OTA, the walker autonomously hybridized with Cy5-labeled DNA and released plenty of Cy5-DNA from the electrode surface with the help of a nicking endonuclease. As a result, the signal of CdS QDs recovered efficiently. As an artificial and popular signal amplification technique, the DNA walking machine greatly improved the sensitivity. Under optimal conditions, the aptasensor not only detected OTA in a linear range from 0.05 nM to 5 nM with a detection limit of 0.012 nM (S/N = 3), but also showed an excellent selectivity for OTA over other mycotoxins.
赭曲霉毒素 A（OTA）对人类和动物的健康构成严重威胁。本文构建了一种简单灵敏的电化学发光（ECL）适体传感器，基于电化学发光共振能量转移（ECL-RET）和切口酶驱动的 DNA 行走机器来检测 OTA。最初，硫化镉半导体量子点（CdS QDs）的信号被 Cy5 有效猝灭。加入 OTA 后，在切口酶的帮助下，行走器自动与 Cy5 标记的 DNA 杂交，并从电极表面释放大量 Cy5-DNA。结果，CdS QDs 的信号得到了有效恢复。作为一种人工且流行的信号放大技术，DNA 行走机器大大提高了灵敏度。在最佳条件下，该适体传感器不仅能够在 0.05 nM 至 5 nM 的线性范围内检测 OTA，检测限低至 0.012 nM（S/N=3），而且对 OTA 具有优异的选择性，对其他霉菌毒素的检测无明显干扰。
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