Resende Danilo Pires, da Costa Adeliane Castro, de Souza Rosa Lorena Pereira, Rodrigues Ana Paula, Santos Annelise Silva E Alvez, Cardoso Camila Kellen, Sousa Jaqueline Danesio, Kipnis André, Silveira Erika Aparecida, Junqueira-Kipnis Ana Paula
Laboratório de Imunopatologia das Doenças Infecciosas, Instituto de Patologia Tropical e Saúde Pública, Universidade Federal de Goiás, Rua 235, Setor Universitário, Goiânia, Goiás, CEP 74605050 Brazil.
Hospital das Clínicas- Programa de Pós-Graduação em Ciências da Saúde, Faculdade de Medicina- UFG, Goiânia, GO, Brazil; Primeira avenida, Setor Universitário, Goiânia, Goiás, CEP 75605020 Brazil.
Tuberculosis (Edinb). 2019 Jan;114:30-41. doi: 10.1016/j.tube.2018.11.003. Epub 2018 Nov 5.
Severe obesity and diabetes lead to a significant decrease in quality of life. Although controversial, population-wide studies have implicated obesity in the development of tuberculosis (TB). Non-classical monocytes have been described in obesity and TB, whereas in diabetes they have been associated with poorer clinical outcomes. The present study focuses on the functional significance of several monocyte populations of obese, obesity-related diabetic (OBDM), non-obese/diabetic tuberculosis and non-obese healthy control patients. Monocytes were evaluated by measuring expression of CD86, CD206, TLR-2 and TLR-4 as well as production of IL-6, IL-12, and by using a mycobacterial growth inhibition assay for both Mycobacterium tuberculosis and M. abscessus subsp. massiliense. Non-classical monocytes from OBDM and non-obese TB patients exhibited similar activation profiles (CD86/CD206/TLR-2 and TLR-4 expressions). Only monocytes from TB patients had a higher positivity for IL-12 and IL-6, whereas adiponectin serum levels increased similarly between TB and OBDM patients. Monocytes from active TB patients and OBDM were more permissive to Mtb growth than obese individuals, but this susceptibility was not observed for M. abscessus subsp. massiliense. From these findings, we conclude that diabetes and tuberculosis had similarities in the population of circulating non-classical monocytes, improving our understanding of the association of these diseases.
重度肥胖和糖尿病会导致生活质量显著下降。尽管存在争议，但全人群研究表明肥胖与结核病（TB）的发生有关。肥胖和结核病患者中已发现非经典单核细胞，而在糖尿病患者中，它们与较差的临床结局相关。本研究聚焦于肥胖、肥胖相关糖尿病（OBDM）、非肥胖/糖尿病结核病患者以及非肥胖健康对照患者的几种单核细胞群体的功能意义。通过测量CD86、CD206、TLR - 2和TLR - 4的表达以及IL - 6、IL - 12的产生，并对结核分枝杆菌和脓肿分枝杆菌亚种马赛亚种进行分枝杆菌生长抑制试验来评估单核细胞。OBDM患者和非肥胖结核病患者的非经典单核细胞表现出相似的激活谱（CD86/CD206/TLR - 2和TLR - 4表达）。只有结核病患者的单核细胞IL - 12和IL - 6阳性率更高，而结核病患者和OBDM患者的血清脂联素水平升高相似。活动性结核病患者和OBDM患者的单核细胞比肥胖个体对结核分枝杆菌的生长更敏感，但对脓肿分枝杆菌亚种马赛亚种未观察到这种易感性。基于这些发现，我们得出结论，糖尿病和结核病在循环非经典单核细胞群体方面存在相似性，这增进了我们对这些疾病关联的理解。
