Clarke G N
Department of Pathology, Royal Women's Hospital, Carlton, Victoria, Australia.
Am J Reprod Immunol Microbiol. 1988 Oct;18(2):44-6. doi: 10.1111/j.1600-0897.1988.tb00233.x.
A total of 41 sera submitted for routine sperm antibody screening were tested by both the indirect immunobead test (IBT) against viable sperm as antigen, and an enzyme-linked immunosorbent assay (ELISA) using the supernatant fraction from washed, sonicated sperm as antigen. Sixteen sera were positive by either IBT or ELISA, but none were positive by both tests. It was concluded that the tests may be detecting antibodies reacting with distinct antigen subgroups, possibly because the ELISA may be detecting antibodies directed against internal antigens or non-sperm-specific antigens such as HLA.
共有41份提交进行常规精子抗体筛查的血清样本，采用以活精子为抗原的间接免疫珠试验（IBT）和以洗涤、超声处理后的精子上清液为抗原的酶联免疫吸附测定（ELISA）进行检测。16份血清样本通过IBT或ELISA检测呈阳性，但两种检测均呈阳性的样本一份也没有。得出的结论是，这两种检测可能检测到与不同抗原亚组发生反应的抗体，这可能是因为ELISA可能检测到针对内部抗原或非精子特异性抗原（如HLA）的抗体。
