Young L G, Smithwick E B
Department of Physiology, Emory University School of Medicine, Atlanta, Georgia.
Am J Reprod Immunol. 1991 Jan;25(1):41-7. doi: 10.1111/j.1600-0897.1991.tb01062.x.
Human sera were identified as positive or negative for sperm-reactive antibodies in a solid-phase enzyme-linked immunosorbent assay (ELISA). Of these, 28 positive and 22 negative sera were blind-coded and used as first antibody to compare three immunoassays, a modified liquid-phase indirect immunobead assay (IBA); a liquid-phase indirect immunofluorescence assay (IFA); and a solid-phase indirect immunogold assay (IGA). These three immunoassays perform both as sperm-reactive antibody detection assays and as sperm-associated antigen localization assays. As antibody detection assays, the IBA, IFA, and IGA gave 37, 27, and 28 positives and 13, 23, and 22 negatives, respectively. The usefulness of the IBA as an antigen localization assay was limited by the size of the marker, while the smaller IFA and IGA markers enabled increased resolution of binding patterns of sperm-reactive antibodies to surface-associated sperm antigens. Although the antigen-antibody binding patterns were almost identical for IFA and IGA, suggesting the same sperm-associated antigens were detected by both assays, the IGA reaction product was stable, higher in resolution, and visible by light microscopy.
在固相酶联免疫吸附测定（ELISA）中，人血清被鉴定为精子反应性抗体阳性或阴性。其中，28份阳性血清和22份阴性血清进行盲编码，并用作一抗，以比较三种免疫测定方法：改良液相间接免疫珠测定法（IBA）；液相间接免疫荧光测定法（IFA）；以及固相间接免疫金测定法（IGA）。这三种免疫测定方法既可用作精子反应性抗体检测测定法，也可用作精子相关抗原定位测定法。作为抗体检测测定法，IBA、IFA和IGA分别给出了37份阳性、27份阳性和28份阳性，以及13份阴性、23份阴性和22份阴性。IBA作为抗原定位测定法的实用性受到标记物大小的限制，而较小的IFA和IGA标记物能够提高精子反应性抗体与表面相关精子抗原结合模式的分辨率。尽管IFA和IGA的抗原-抗体结合模式几乎相同，表明两种测定法检测到的是相同的精子相关抗原，但IGA反应产物稳定，分辨率更高，并且在光学显微镜下可见。
