Cann J R, Hinman N D
Biochemistry. 1976 Oct 19;15(21):4614-22. doi: 10.1021/bi00666a011.
A mass transport theory of the gel chromatographic procedure of Hummel and Dreyer for measuring the binding of small ligand molecules to macromolecules has been formulated for ligand-mediated macromolecular association. It is concluded that the Hummel-Dreyer procedure is the method of choice for quantitating ligand binding in such systems. Guidelines are drawn for unambiguous interpretation of the nonclasical elution profiles in terms of the number of binding sites on the macromonomer and their intrinsic binding constant. Most of these guidelines also apply to static equilibrium methods such as equilibrium dialysis.
针对配体介导的大分子缔合，已为Hummel和Dreyer用于测量小配体分子与大分子结合的凝胶色谱程序制定了一种质量传输理论。得出的结论是，Hummel-Dreyer程序是定量此类系统中配体结合的首选方法。根据大分子单体上结合位点的数量及其固有结合常数，为明确解释非经典洗脱曲线制定了指导原则。这些指导原则中的大多数也适用于诸如平衡透析等静态平衡方法。
