Center of Reproductive Medicine, Guangzhou Women and Children's Medical Center, Guangzhou Medical University, China.
Adv Clin Exp Med. 2019 Apr;28(4):523-527. doi: 10.17219/acem/91010.
In thawed embryo transfer cycles, the most common method is to transfer the embryos after 2 h of culture. Clinical outcomes of frozen-thawed cleavage embryo transfer cycles regarding the embryos status and the culture time of frozen-thawed cleavage embryos were limited and did not elucidate all unclear issues.
The objective of this study was to examine the clinical outcomes of frozen-thawed cleavage embryo transfer cycles according to the embryos status and the culture time (2 h or overnight).
In this retrospective study (5-year period), 1,654 frozen-thawed embryos were analyzed. Firstly, frozen-thawed cleavage embryos were divided into 2 groups according to their status as follows: with at least 1 optimal embryo and without optimal embryos. Secondly, both of them were divided into 2 groups according to the culture time (2 h or overnight). Age of the female, infertility factors, clinical pregnancy, implantation rate, and live birth rate were compared.
There were no statistically significant differences in the pregnancy rate, the implantation rate, live birth rate, the miscarriage rate, and the ectopic pregnancy rate in each group. However, the implantation rate increased after 2 h of incubation (41.1%) compared to overnight incubation (36.0%) in the group with at least 1 optimal day-3 embryo (p < 0.05). The cancellation rate in the suboptimal day-3 embryos group (9.1%) was higher than in the group containing at least 1 optimal embryo (0.2%) for the long (overnight) culture (p < 0.05).
The implantation rate can be improved in the optimal day-3 embryos transferred after 2 h of culture, but not for suboptimal day-3 embryos. Some unnecessary transfers can be avoided after overnight culture because of no further cleavage of the embryos.
在解冻胚胎移植周期中，最常见的方法是在培养 2 小时后移植胚胎。关于冷冻-解冻卵裂胚胎移植周期的临床结局，冷冻-解冻卵裂胚胎的状态和培养时间的相关研究有限，并未阐明所有不明确的问题。
本研究旨在根据胚胎状态和培养时间（2 小时或过夜）检查冷冻-解冻卵裂胚胎移植周期的临床结局。
在这项回顾性研究（5 年期间）中，分析了 1654 个冷冻-解冻胚胎。首先，根据冷冻-解冻卵裂胚胎的状态将其分为 2 组：至少有 1 个优质胚胎和无优质胚胎。其次，根据培养时间（2 小时或过夜）将它们均分为 2 组。比较了女性年龄、不孕因素、临床妊娠、种植率和活产率。
每组的妊娠率、种植率、活产率、流产率和宫外孕率均无统计学差异。然而，在至少有 1 个优质第 3 天胚胎的组中，培养 2 小时后的种植率（41.1%）高于过夜培养（36.0%）（p<0.05）。在第 3 天胚胎质量不佳的组（9.1%）中，过夜培养的取消率（9.1%）高于含有至少 1 个优质胚胎的组（0.2%）（p<0.05）。
在培养 2 小时后移植优质第 3 天胚胎可以提高种植率，但对于质量不佳的第 3 天胚胎则不然。由于胚胎没有进一步分裂，过夜培养后可以避免一些不必要的移植。
