Song Guiqin, He Lang, Yang Xiaolin, Yang Yan, Cai Xiaoming, Liu Kang, Feng Gang
Institute of Tissue Engineering and Stem Cells, Nanchong Central Hospital, the Second Clinical Medical College, North Sichuan Medical College, Nanchong, Sichuan, P.R. China.
Department of Biology, North Sichuan Medical College, Nanchong, Sichuan, P.R. China.
J Int Med Res. 2020 Jan;48(1):300060518815364. doi: 10.1177/0300060518815364. Epub 2019 Feb 3.
It has been reported that 80% of all breast carcinoma cases are invasive ductal carcinoma (IDC), and 45% to 78% of invasive breast carcinoma cases are associated with ductal carcinoma (DCIS). Therefore, it is important to gain insights into transcriptome changes that occur during DCIS progression to IDC.
We downloaded Gene Expression Omnibus databases GSE21422 and GSE3893, and performed differentially expressed gene (DEG) analysis and cluster analysis, followed by pathway enrichment analysis and Oncomine analysis.
Twenty-six conserved DEGs were identified in both GSE21422 and GSE3893. These genes are mainly enriched in intermediate filament-based processes, immune responses, infection response, and phagosomes. Among them, , , , and were reported to be involved in DCIS progression to IDC. High expression of , , and in different types of breast cancer was validated using different Oncomine datasets. Moreover, elevated and levels were associated with breast cancer recurrence. Importantly, the overexpression of was correlated with breast cancer metastasis.
This study revealed the molecular characteristics associated with progression from DCIS to IDC. It also identified potential biomarkers for DCIS progression to IDC, which will aid breast cancer diagnosis and prevention.
据报道，所有乳腺癌病例中有80%为浸润性导管癌（IDC），45%至78%的浸润性乳腺癌病例与导管原位癌（DCIS）相关。因此，深入了解DCIS进展为IDC过程中发生的转录组变化非常重要。
我们下载了基因表达综合数据库GSE21422和GSE3893，并进行了差异表达基因（DEG）分析和聚类分析，随后进行通路富集分析和Oncomine分析。
在GSE21422和GSE3893中均鉴定出26个保守的DEG。这些基因主要富集于基于中间丝的过程、免疫反应、感染反应和吞噬体。其中，据报道， 、 、 和 参与了DCIS向IDC的进展。使用不同的Oncomine数据集验证了 、 和 在不同类型乳腺癌中的高表达。此外， 和 水平升高与乳腺癌复发相关。重要的是， 的过表达与乳腺癌转移相关。
本研究揭示了与DCIS进展为IDC相关的分子特征。它还确定了DCIS进展为IDC的潜在生物标志物，这将有助于乳腺癌的诊断和预防。
