Pérez-Núñez Daniel, Sunwoo Sun-Young, Sánchez Elena G, Haley Nicholas, García-Belmonte Raquel, Nogal Marisa, Morozov Igor, Madden Daniel, Gaudreault Natasha N, Mur Lina, Shivanna Vinay, Richt Juergen A, Revilla Yolanda
CBMSO-CSIC-UAM, C/ Nicolás Cabrera 1, Campus de Cantoblanco, Madrid, 28049, Spain.
Department of Diagnostic Medicine & Pathobiology, College of Veterinary Medicine, Kansas State University, K218 Mosier hall, 1800 Denison Ave, Manhattan, KS, 66506, USA.
Vet Immunol Immunopathol. 2019 Feb;208:34-43. doi: 10.1016/j.vetimm.2018.11.018. Epub 2018 Dec 21.
African swine fever virus (ASFV) causes serious disease in domestic pigs for which there is no vaccine currently available. ASFV is a large DNA virus that encodes for more than 150 proteins, thus making the identification of viral antigens that induce a protective immune response difficult. Based on the functional roles of several ASFV proteins found in previous studies, we selected combinations of ASFV recombinant proteins and pcDNAs-expressing ASFV genes, to analyze their ability to induce humoral and cellular immune responses in pigs. Pigs were immunized using a modified prime-boost approach with combinations of previously selected viral DNA and proteins, resulting in induction of antibodies and specific cell-mediated immune response, measured by IFN-γ ELISpots. The ability of antibodies from pigs immunized with various combinations of ASFV-specific antigens to neutralize infection in vitro, and antigen-specific activation of the cellular immune response were analyzed.
非洲猪瘟病毒（ASFV）可致使家猪患上严重疾病，目前尚无针对该病的疫苗。ASFV是一种大型DNA病毒，可编码150多种蛋白质，因此难以鉴定能引发保护性免疫反应的病毒抗原。基于先前研究中发现的几种ASFV蛋白的功能作用，我们选择了ASFV重组蛋白与表达ASFV基因的质粒DNA的组合，以分析它们在猪体内诱导体液免疫和细胞免疫反应的能力。采用改良的初免-加强免疫方法，用先前选定的病毒DNA和蛋白质组合对猪进行免疫，通过干扰素γ酶联免疫斑点法检测，结果诱导出了抗体和特异性细胞介导免疫反应。分析了用ASFV特异性抗原的各种组合免疫的猪所产生的抗体在体外中和感染的能力，以及细胞免疫反应的抗原特异性激活情况。
