Dhar R, Lastimoza J L, Hira P R
Microbiology Section, Al-Adan Hospital, Fahaheel, Kuwait.
Diagn Microbiol Infect Dis. 1988 Dec;11(4):189-94. doi: 10.1016/0732-8893(88)90002-8.
We adapted the conventional indirect fluorescent antibody (IFA) test to assay IgM and IgG Brucella-specific antibodies to differentiate acute from chronic infections rather than measure total antihuman globulin specific antibodies. The results were compared with the slide agglutination test (SAT) used for screening and the quantitative microagglutination test (MAT). Of a total of 118 randomly selected samples sent for anti-Brucella antibodies received at a general hospital laboratory, 58 (47.9%) were found to be positive for IFA-IgG test but not necessarily by other tests. Eleven of these cases were positive for Brucella melitensis by culture. Sixty serum samples found negative for Brucella antibodies by IFA and other tests were of patients with medical conditions other than brucellosis. Fifty serum samples from healthy blood donors were negative for Brucella spp. antibodies by all the three tests. The IFA test was found to be a more sensitive test than MAT and distinguished an acute infection from chronic disease.
我们对传统间接荧光抗体（IFA）检测方法进行了改良，以检测布鲁氏菌特异性IgM和IgG抗体，从而区分急性感染和慢性感染，而非检测总抗人球蛋白特异性抗体。将结果与用于筛查的玻片凝集试验（SAT）和定量微量凝集试验（MAT）进行比较。在一家综合医院实验室收到的总共118份随机选取的用于检测抗布鲁氏菌抗体的样本中，58份（47.9%）IFA-IgG检测呈阳性，但其他检测不一定呈阳性。其中11例经培养证实为羊种布鲁氏菌阳性。60份经IFA和其他检测布鲁氏菌抗体呈阴性的血清样本来自患有除布鲁氏菌病以外其他疾病的患者。50份来自健康献血者的血清样本经所有三项检测均未检测到布鲁氏菌属抗体。结果发现，IFA检测比MAT检测更敏感，且能区分急性感染和慢性疾病。
