Gad El-Rab M O, Kambal A M
Department of Pathology, College of Medicine, King Khalid University Hospital, King Saud University, Riyadh, Saudi Arabia.
J Infect. 1998 Mar;36(2):197-201. doi: 10.1016/s0163-4453(98)80013-3.
An ELISA test for IgG and IgM antibrucella antibodies was found to be effective in diagnosis of human brucellosis. Assays for IgG and IgM in 30 culture-positive cases gave significant ELISA values. By the standard agglutination test, 10% of these cases gave readings less than 1:160. These are considered insignificant, taking 1:160 as the accepted cut-off value. Moreover, in an extra 135 samples from suspected brucella cases, where only serology was requested (77.6% of all cases), 7.4% were found to have IgM brucella antibodies by ELISA. In all of these, the corresponding agglutination titres were less than 1:80 and hence reported as insignificant. We report the detection of IgG and IgM antibodies in samples from patients with both acute and chronic disease. In few patients with acute disease, only IgM was detected. These findings are discussed in comparison with earlier studies. Finally, the ELISA test, in addition to measuring antibody classes directly, also detects incomplete antibodies. By this, it can efficiently replace the 2 mercaptoethanol test (2ME) and the Coomb's antihuman-globulin test. This saves considerable laboratory cost and time.
一种用于检测IgG和IgM抗布鲁氏菌抗体的ELISA试验被发现对人类布鲁氏菌病的诊断有效。对30例培养阳性病例的IgG和IgM检测得出了显著的ELISA值。通过标准凝集试验，这些病例中有10%的读数低于1:160。以1:160作为公认的临界值，这些读数被认为无意义。此外，在另外135份来自疑似布鲁氏菌病例的样本中（仅要求进行血清学检测，占所有病例的77.6%），通过ELISA发现7.4%的样本含有IgM布鲁氏菌抗体。在所有这些样本中，相应的凝集效价均低于1:80，因此报告为无意义。我们报告了在急性和慢性疾病患者样本中检测到IgG和IgM抗体。在少数急性病患者中，仅检测到IgM。将这些发现与早期研究进行了比较讨论。最后，ELISA试验除了能直接检测抗体类别外，还能检测不完全抗体。通过这种方式，它可以有效地替代2-巯基乙醇试验（2ME）和库姆斯抗人球蛋白试验。这节省了可观的实验室成本和时间。
