Department of Epidemiology and Statistics, School of Public Health, Jiangsu Key Laboratory and Translational Medicine for Geriatric Disease, Medical College of Soochow University, Suzhou, Jiangsu, China.
Suzhou Industrial Park Centers for Disease Control and Prevention, Suzhou, China.
J Clin Lab Anal. 2019 May;33(4):e22842. doi: 10.1002/jcla.22842. Epub 2019 Feb 4.
Although recent studies have indicated that gut microbiome dysbiosis was significantly associated with the onset of type 2 diabetes mellitus (T2DM), information on the role of blood microbiome in T2DM development is scarce.
Fifty incident T2DM cases and 100 matched non-T2DM controls were selected from a prospective cohort study of "135." The composition of the blood microbiome was characterized using bacterial 16S ribosomal RNA (16S rRNA) gene sequencing from pre-diagnostic blood sample. The amplicons were normalized, pooled, and sequenced on the Illumina MiSeq instrument using a MiSeq Reagent Kit PE300 v3 kit.
Totally, 3 000 391 and 6 244 227 high-quality sequences were obtained from T2DM patients and non-T2DM controls, respectively. The mean diversity of the blood microbiome (Simpson, Chao1 and Shannon indices) was not different between two groups at baseline. At genus level, the Aquabacterium, Xanthomonas, and Pseudonocardia were presented with lower abundance, while Actinotalea, Alishewanella, Sediminibacterium, and Pseudoclavibacter were presented with higher abundance among T2DM cases compared to those in non-T2DM controls. As the results shown, participants carried the genus Bacteroides in blood were significantly associated with a decreased risk for T2DM development, with 74% vs 88% (adjusted OR: 0.367, 95% CI: 0.151-0.894). However, participants carried the genus Sediminibacterium have an increased risk for T2DM, with adjusted OR (95% CI) being 14.098 (1.358, 146.330).
Blood microbiome may play an etiology role in the development of T2DM. These findings would be useful to develop microbiota-based strategies for T2DM prevention and control.
尽管最近的研究表明肠道微生物群失调与 2 型糖尿病（T2DM）的发生显著相关，但血液微生物群在 T2DM 发展中的作用信息仍然缺乏。
从“135”前瞻性队列研究中选择了 50 例新诊断的 T2DM 病例和 100 例匹配的非 T2DM 对照。使用细菌 16S 核糖体 RNA（16S rRNA）基因测序从预诊断血样中描述血液微生物组的组成。将扩增子进行归一化、合并，并使用 MiSeq Reagent Kit PE300 v3 试剂盒在 Illumina MiSeq 仪器上进行测序。
从 T2DM 患者和非 T2DM 对照中分别获得了 3000391 和 6244227 条高质量序列。两组在基线时血液微生物组的多样性（辛普森、Chao1 和香农指数）没有差异。在属水平上，与非 T2DM 对照组相比，Aquabacterium、Xanthomonas 和 Pseudonocardia 的丰度较低，而 Actinotalea、Alishewanella、Sediminibacterium 和 Pseudoclavibacter 的丰度较高。结果表明，血液中携带拟杆菌属的参与者发生 T2DM 的风险显著降低，分别为 74%和 88%（调整后的 OR：0.367，95%CI：0.151-0.894）。然而，携带 Sediminibacterium 属的参与者发生 T2DM 的风险增加，调整后的 OR（95%CI）为 14.098（1.358，146.330）。
血液微生物群可能在 T2DM 的发展中起病因作用。这些发现有助于开发基于微生物组的 T2DM 预防和控制策略。
