Hai Chao, Gong Hongyu, Xu Yanan, Liu Xuefei, Bai Chunling, Su Guanghua, Yang Lei, Li Guangpeng
State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010070, China.
Vet Sci. 2025 Jun 7;12(6):560. doi: 10.3390/vetsci12060560.
The gut-muscle axis plays a vital role in host metabolism and health. Although the gene is a well-known negative regulator of muscle growth, its role in intestinal function and metabolism remains unclear. Understanding this connection is crucial for revealing the systemic impact of gene editing and its potential to improve metabolic efficiency in livestock. In this study, we investigated the influence of deletion on gut microbiota composition and carbohydrate metabolism in the cecum and colon of cattle. Using integrated metagenomic, metabolomic, serum biochemical, and muscle transcriptomic analyses, we found significant alterations in microbial communities and key metabolic pathways. and in the colon, as well as in the cecum, were closely linked to carbohydrate metabolism. Differential microbes and metabolites influenced key metabolic pathways, including glycolysis/gluconeogenesis and lipopolysaccharide biosynthesis. Functional gene analysis identified as the most critical bacterium affecting glycolysis/gluconeogenesis. Additionally, genes related to carbohydrate esterases were upregulated. These changes correlated with reduced serum glucose and insulin levels while increasing muscle gene expression related to glucose-to-lactose conversion. Overall, gene editing alters gut microbiota composition and carbohydrate metabolism in the cecum and colon, thereby influencing host glucose metabolism and energy homeostasis.
肠-肌肉轴在宿主代谢和健康中起着至关重要的作用。尽管该基因是众所周知的肌肉生长负调节因子，但其在肠道功能和代谢中的作用仍不清楚。了解这种联系对于揭示基因编辑的系统影响及其提高家畜代谢效率的潜力至关重要。在本研究中，我们调查了基因缺失对牛盲肠和结肠中肠道微生物群组成和碳水化合物代谢的影响。通过综合宏基因组学、代谢组学、血清生化和肌肉转录组学分析，我们发现微生物群落和关键代谢途径有显著改变。结肠中的和，以及盲肠中的，都与碳水化合物代谢密切相关。差异微生物和代谢产物影响关键代谢途径，包括糖酵解/糖异生和脂多糖生物合成。功能基因分析确定为影响糖酵解/糖异生的最关键细菌。此外，与碳水化合物酯酶相关的基因上调。这些变化与血清葡萄糖和胰岛素水平降低相关，同时增加了与葡萄糖向乳糖转化相关的肌肉基因表达。总体而言，基因编辑改变了盲肠和结肠中的肠道微生物群组成和碳水化合物代谢，从而影响宿主葡萄糖代谢和能量稳态。
